Experimental Study Of The Bifidobacterium Vinegar Effection In The Rats,Prophylactic High-fat And Obesity

Objective:Study on the effect on prophylactic high-fat and obesity and possiblemechanism of bifidobacterium vinegar in rat.Methods:1. Experimental study of the bifidobacterium vinegar affect the weightof rat fed with high-fat diet:48SD rats were randomly divided into6groups: bifidobacterium vinegar groups of three different doses:low,medium and high doses groups, ordinary vineger group, obesity modelgroup and basic group.Feeding on ordinary food rat with administration bygavage. To feed with high-fat diet on rat,except basic group.The level ofBody weight, body fat, adipocyte diameter, Lee′s index, leptin and insuline.2. The influence of the bifidobacterium vinegar on the plasama lipid ofrat fed with high-fat diet:48SD rats were randomly divided into6groups:bifidobacterium vinegar groups of three different doses:low, medium andhigh doses groups, ordinary vineger group, hyperlipemia model group andbasic group.Feeding on ordinary food rat with administration by gavage. To feed with high-fat diet on rat,except basic group. Body weight, body fatindex, fatty degeneration in liver were determined3. The study on the mechanism of bifidobacterium vinegar strainsaffecting to the plasama lipid of rat fed with high-fat diet:48SD rats wererandomly divided into6groups: bifidobacterium vinegar groups of threedifferent doses:low, medium and high doses groups, ordinary vineger group,hyperlipemia model group and basic group.Feeding on ordinary food ratwith administration by gavage. To feed with high-fat diet on rat,exceptbasic group. Measured the lipidoses in liver，mRNA expression of LDLR、HMGCoA reductase in liver tissue， Immunohistochemistry analysisemployed to observe the expression of LDLR in liver.Results:1. Compared with control groups, The level of Body weight, body fat,adipocyte diameter, Lee′s index, leptin and insuline.were improvedobviously. particularly, leptin and insuline.2. Compared with control groups, The level of Body weight, body fatindex, fatty degeneration in liver were determined were improvedobviously.particularly, TG and HDL-C.3. Bifidobacterium vinegar can adjust to the LDLR and HMGCoAreductase in liver from increasing with some degree.Conclusion:Bifidobacterium vinegar have effect on preventing the rat of high-fat and obesity from increasing with some degree, had no effect on.