Study On IL28B Gene Polymorphism And Prognostic Factors Of Hepatitis C Patients

Objective:Investigating the prognostic factors of hepatitis C and analyzing therelationship of the prognosis of hepatitis C with IL28B polymorphism, in order toprovide a scientific information for conducting treatment, improving outcomes andpromoting rehabilitation.Methods:Patients with hepatitis C were enrolled in this historical cohort study. Theself-designed epidemiological questionnaire was used to investigate the generalinformation, medical history, life style and so on. All patients can be divided into twogroups, poor prognosis group and good prognosis group, based on the judgmentcriterion of prognosis. χ2test and logistic regression analysis were used to filtrate theinfluencing factors on the prognosis of hepatitis C. IL28B (rs12979860) genotypingwas performed using the Pyrosequencing. The differences of allele and genotypefrequencies among the poor prognosis group and good prognosis group werecompared. In order to assess the correlation between IL28B (rs12979860) and theprognosis of hepatitis C.Results:1.Significant difference in the prevalence of poor prognosis was not found amongdifferent groups of smoking, drinking tea and marital status(P>0.05). The oppositeresult was observed in gender, occupation, obesity, hepatitis B history, treatment andHCV genotype (P<0.05).2.The trend χ2test results showed that the family economic status was notsignificantly associated with prognosis of hepatitis C(P>0.05). And age, alcoholconsumption, education level, physical activity and HCV-RNA titers were significantly associated with prognosis of hepatitis C(P<0.05).3.The distribution of IL28B rs12979860genotype: There were three genotypes (CC,T/C and TT) in IL28B (rs12979860). Among203patients with hepatitis C,169individuals had CC homozygote-es (83.25%), CT32(15.76%), and TT2(0.98%).4.Through χ2test, data showed that the distribution of allele (C, T) of IL28B(rs12979860) was associated with the prognosis（P=0.002）. And the prevalence ofpoor prognosis among patients with T allele was higher than those with C allele(RR=1.547).5.IL28B rs12979860genotype and HCV prognosis: the CC genotype carriers were169cases, poor prognosis and good prognostic patients were respectively81and88.The prevalence of poor prognosis was47.93%; the CT genotype carriers were32cases, poor prognosis and good prognostic patients were respectively26and6.Theprevalence of poor prognosis was81.25%; the TT genotype carriers was2cases, bothwith poor prognosis. Statistically significant difference could be detected betweendifferent IL28B genotypes (P=0.002).6.Multi-factor non-conditional Logistic regression analysis results showed that theinfluence factors of prognosis of chronic hepatitis C were obesity (P<0.001,RR=4.031), history of hepatitis B (P<0.001, RR=8.431), age (P<0.001, RR=6.197),treatment (P<0.001, RR=0.287), alcohol (P<0.001, RR=9.244), physical exercise(P<0.001, RR=0.385), kinship care (P<0.001, RR=0.430),HCV genotype (P<0.001,RR=14.041)and IL28B rs12979860genotype(P<0.001, RR=10.433).Conclusions:1.The relationship was close between IL28B gene polymorphism and prognosis ofhepatitis C. The T allele was the poor prognostic markers of hepatitis C. The patientswho carried CC genotype have better prognosis.2.Age, Obesity, Hepatitis B, Drinking and HCV genotype were influencing factors ofprognosis of the hepatitis C. Antiviral therapy, Physical exercise and Kinship carewere the protective factors, which can promote improving the prognosis of the hepatitis C patients.