Different Size Of Rhubarb Powder Quality Standard And Pharmacokinetic Study

Object i ve The aim of this project is to establish quality standards for different size powder of rhubarb, rhubarb different particle size powders in rats in vivo pharmacokinetic behavior, bioavailability differences, explore various particle size of rhubarb powder in the pharmacokinetics of the impact of the dynamics.Methods Rhubarb (Rheum palmatum L.) powder of80mesh,120mesh,200mesh and400mesh and so on four different particle size, their traits, physical and chemical, TLC identification and Rumex glycoside Loss on drying, total ash, water-soluble extract were examined. Content determination by high performance liquid UV chromatography detection wavelength to254nm with methanol and0.1%phosphoric acid (85:15) as mobile phase, to develop a standard curve of rhubarb five anthraquinone compounds, calculation of the four kinds of the total content of the five anthraquinones in the particle size of rhubarb powder extract.On Rats were two kinds of200mesh and400mesh powder, mesh inner canthus blood blood was collected at set time points, after a certain approach, using high performance liquid chromatography, fluorescence detector, methanol and0.1%phosphoric acid (85:15) as mobile phase; reference wavelength:440nm; detection wavelength:520nm; determination of plasma rhubarb four anthraquinone compounds content (the physcion in plasma detected some point in time, but because the content is very low, has exceeded the limit of detection, so here only detect four anthraquinones), draw the rhubarb blood drug concentration-time curve, using the3P97software to calculate the pharmacokinetic parameters. This experiment attempt by HPLC-MS method for determination of plasma concentration of rhubarb in rats, to explore new methods of pharmacokinetic studies in rhubarb. Results Four different particle size of rhubarb powder in traits, physical and chemical, TLC, Rumex glycosides, loss on drying, total ash, water-soluble extracts of the experimental results are in line with the relevant provisions of the2010edition of the "Chinese Pharmacopoeia" in the content measuring aspects of rhubarb five anthraquinones:Aloe emodin, rhein, emodin, chrysophanol, emodin ether standard curve equation as follows:y=5.9423x+351.6, y=67.703x-3.7837, y=75.105x-4.6878, y=75.105x-4.6878, y=49.006x-3.0415. Aloe-emodin, rhein, emodin, chrysophanol reference substance the0.8μg/ml～8μg/ml concentration range; physcion reference substance in the0.4μg/ml～4μg/ml concentration range, the concentration and peak area a good linear relationship. Four kinds of powder assay results are as follows:1.94%,2.18%,2.31%,3.07%.In large rat poison on behalf of the kinetic experiments, using the3P97software to calculate the200mesh and400mesh two kinds of rhubarb powder in rats in vivo pharmacokinetic parameters, the four anthraquinone compounds basically are about2h to reach the maximum plasma concentration value, and400mesh powder, Cmax Cmax than200mesh powder to a large biological degrees than were:1.51,1.76,1.07,1.91, more than1times. In addition, the plasma concentration of rats in the HPLC-MS detection, the in vitro samples, including standards and Radix experimental results, but not detected in vivo samples of plasma and urine samples excimer ion peak of the target composition.Conclusion The initial establishment of quality standards of different size powder of rhubarb, the pharmacokinetic behavior of the different size of rhubarb powder. Can draw conclusions from the above results:traditional Chinese medicine to crush the different levels, there are certain quality standards and pharmacokinetics of traditional Chinese medicine grinding technology to improve efficiency in the use of traditional Chinese medicine to save the traditional Chinese medicine resources of great significance, the the conclusion of the subject of traditional Chinese medicine in the production and clinical applications have played a certain reference.Quality standards by the assay results can be seen that With crushed increasing herbs crushed particle size decreases, the target of extraction and content continues to increase, indicating that increasing the degree of comminuted help to increase the dissolution of ingredients and the extraction rate; in terms of pharmacokinetics, increasing the herbs crushing can increase Cmax and bioavailability of the drug, can be seen in clinical use, the crushing increases herbs help to reduce the dosage, to improve efficacy.