The Induction Of Apoptosis In Human Gastric Cancer SGC-7901Cells And Human Lung Cancer A549Cells Through Upregulation Bax And Downregulation Bcl-2Gene By ZTO

Apoptosis plays an important role to maintain the normal morphology andfunction of tissues and organs, it is closely related with tumor occurrence anddevelopment of great significance in the treatment of tumors.Chinese herbal medicine with toxic side effects, the stability of the treatmentcharacteristics, therefore, screening new anticancer drugs in Chinese herbal medicinehas become a hot topic. Curcuma oil has antibacterial, anti-inflammatory, anti-cancerrole in the anti-tumor has broad application prospects.AIM:To use the human gastric cancer SGC-7901cells cells for research,exploring the anti-tumor effect of zedoary turmerie oil on the human gastric cancerSGC-7901cells and human lung cancer A549cells and the apoptosis-related genes ofbax/bcl-2eapression.METHODS: To observe the cell morphology of the zedoary turmerie oil onthe human gastric cancer SGC-7901cells and human lung cancer A549cells underthe inverted microscope in different concentrations and at24h,48h and72h. Detectionof the cell viability of the zedoary turmerie oil on the SGC-7901cells and A549cells by trypan blue-rejected staining method and determined the IC50value. The flowcytometry is used to investigate the changes of the cell cycle distribution and themitochondrial membrene potential,and the combination of Annexin V-FITC/PImethod to detect apoptosis. Real time RT-PCR detection of bax, bcl-2mRNAexpression levels, Western blot detection of protein expression levels.RESULTS: The zedoary turmerie oil on both the human gastric cancerSGC-7901cells and human lung cancer A549cells for48hours showed varyingdegrees of features of apoptosis and dose-effect relationship. For48hours, thezedoary turmerie oil on SGC-7901cells and A549cells optimal concentration forinduction of apoptosis were110μg/mL and80μg/mL.The IC50values were108.002±2.492μg/mL and77.462±0.681μg/mL. The results of the flow cytometryshow that the detection of the cell cycle,obtained oil of Curcuma mechanism of action of the SGC-7901cells and A549cells cells were arrested in S phase and G2phase andthe mitochondrial membrane potential with increasing concentration decreased. Realtime RT-PCR and Western Blot results show that the SGC-7901cells and A549cellsfor48h can be upregulation bax and downregulation bcl-2gene expression by acertain concentration of Curcuma oil.Conclusion: The induction of apoptosis in SGC-7901cells and A549cellstreated with a certain concentration of Curcuma oil and a certain period of time, canup-regulate bax gene expression and down-regulate bcl-2gene expression inducedapoptosis.