The Changes Of1,25（OH）₂D₃and IL-8in Serum Of The Children With Hashimoto Thyroiditis

Object: To understand the different levels of IL-8and1,25(OH)2D3in serum between the children with HT and normal children, and the changes in their levels before and after treatment. To study the correlation of the levels of IL-8and1,25(OH)2D3with age, FT3, FT4, TSH, TRAb, TPOAb, TGAb. Discuss the roles of IL-8and1,25(OH)2D3in HT’s occurrence and development, provide new thinking and basis for discussing the pathogenesis and treatment of HT in the future.Materials and Methods:Thirty cases of children with HT, which came from Tianjin Medical University General Hospital, pediatric endocrine clinic and without treatment during the year2011-2012. Four boys and26girls were included in the study. All of them were associated with different levels of thyroid enlargement, abnormal thyroid functions. There were no association with other autoimmune disease history, nor any recent infection and history of allergy, except in various diseases and drugs which may affect bone calcium metabolism. The group of children for HT and the normal control group included30children in total. All of them were healthy. The10boys and the30girls, all had no thyroid and other autoimmune disease family history.The function of heart, liver and kidney were all normal. All the children were fasting when the blood sample from the early morning was taken. Adopted light chemical analysis method to detect FT3, FT4, TSH, radiation immune analysis methods were used to detect TGAb, enzyme-linked immune method to detect TRAb, shine immune analysis method to detect TPOAb, ELISA method to detect IL-8and1,25(OH)2D3. All the children with HT were given L-T4for treatment. After3to12months of treatment, clinical symptoms disappear and the thyroid function returned back to normal. Blood samples were taken again to detect the levels of FT3, FT4, TSH, TRAb, TPOAb, TGAb, IL-8and1,25(OH)2D3in the serum. With SPSS16.0statistical software analysis data, the change of all index were compared between the normal group and HT group, HT group before and after treatment for relief, HT group after treatment for relief and the normal group. A statistical significant correlation by the Pearson’s method analysis was done to correlate the IL-8and1,25(OH)2D3with each index. Results:1. By comparing each index between HT group before treatment and normal group, we found that TSH, TPOAb, TGAb, IL-8of HT group were significantly higher than normal group.1,25(OH)2D3of the HT group was significantly lower than the normal group. And the difference had statistical significance, meaning (p<0.001). FT4of HT group was also reduce compared to the normal group, in α=0.05level, the statistically significant difference. But gender, age, FT3, TRAb differences had no statistical significance (p>0.05).2. The HT group was treated by giving L-T4until the thyroid function returned to normal. Compared with before treatment, FT4(p<0.05) and1,25(OH)2D3(p<0.001), significantly increased. TSH and IL-8decreased obviously (p<0.001). TPOAb and TGAb were also lower than before treatment, and the p<0.05, but their level was still higher than the normal group. FT3and TRAb had no changes.3. The HT group was treated by giving L-T4until the thyroid function returned to normal. Compared with normal group FT3, FT4, TSH, TRAb, IL-8,1,25(OH)2D3had no changes, there was no statistical significant difference. After the treatment, although the TGAb and the TPOAb were all obviously decreased than before, they were still higher than the normal group, and had statistically significant difference (p <0.05).4. Through the correlation analysis, we found that the IL-8and1,25(OH)2D3in the serum of children with HT had no relationship with FT3, FT4, TRAb, TGAb. But there was a certain correlation with TSH and TPOAb and IL-8with TSH. TPOAb had a positive correlation, relevant coefficients were (r=0.522and r=0.401, p<0.05) respectively.1,25(OH)2D3with TSH, TPOAb had a negative correlation, relevant coefficients were (r=0.411and r=0.418, p<0.05) respectively.Conclusions:1. The IL-8level in serum of children with HT was obviously higher than normal, and1,25(OH)2D3was lower. After treatment, IL-8and1,25(OH)2D3could all be restored to a normal level. This showed that IL-8and1,25(OH)2D3may be Pathological physiology factor n the process of HT.2. After the correlation analysis we knew that the levels of IL-8and1,25(OH)2D3 in the serum of children with HT had a certain correlation with TSH and TPOAb. IL-8had a positive correlation and1,25(OH)2D3had a negative correlation with the above. The level of IL-8was higher or the level of1,25(OH)2D3was lower, the more the thyroid antibodies produced and the more seriously the thyroid was damaged, the higher the level of TSH. This suggested that, IL-8and1,25(OH)2D3may be involved in the formation of thyroid antibodies.3. After treatment of the children with HT, clinical symptoms disappeared, thyroid function returned to normal, IL-8and1,25(OH)2D3levels were backed to the normal range. Although the TPOAb and the TGAb levels were still higher than normal level, it also declined compared with before treatment, and the difference had statistical significance. This also suggested that if we give the children with HT the right amount of1,25(OH)2D3or its analogues, the drug which could antagonize IL-8role or its receptor, might have certain curative effect to shorten the course of HT, but the exact clinical effects need to be further observed.