| Rhizoma Alismatis was described in herbal contribution of past dynasties, and has a long history to use. It have been used as a diuretic action and was believed to have effect of expelling damp-heat and reducing blood-fat. So far, only one or two triterpenoids in Rhizoma Alismatis were analyzed by quantitative analytical method, with great one-sided. Rhizoma Alismatis as the investigative object in this topic, methodological investigative model of quantitative analysis of four triterpenoids in Rhizoma Alismatis was set up, and was carried out from accuracy, precision, linear correlation; linear range, stability, LOD and LOQ tests. Using high performance liquid chromatography, chromatographic column::Agilent ZORBAX SB-C18Chromatographic column (4.6mm×250mm,5μm), mobile phase for water(A pump)-acetonitrile (B pump), gradient elution:0-5min,30-85%,5-30min,85%,30-35min,85%-100%,35-40min,100%, flow number:1.0min/mL, ultraviolet detectors, detectable wavelength:210nm, temperature of chromatographic column to room temperature. In this chromatographic conditions, undertake methodological investigation of quantitative analysis of four triterpenoids in Rhizoma Alismatis by HPLC method, and the HPLC method was applied for determining content of alisol A24-acetate, alisol B, alisol B23-acetate and11-deoxyalisol B23-acetateby. The equation of linear regression were y=0.6318x+0.0952, y=0.4894x+0.1956, y=0.5319x+0.1893, y=0.4255x+0.6690respectively; the linear range were0.33-33.06μg/ml0.39-34.32μg/ml,1.29-129.60μg/ml,1.14-98.80ug/ml respectively; the average recovery were94.8±3.81%,96.4±4.22%,93.6±6.72%,96.4±5.33%respectively; the precision of intra-and inter-days:RSD<10%; the stability:RSD <9.0%. The results showed that the assay mehod are simple, accurate and with good reproducibility and provide a quantitative basis for the quantitative analysis of Rhizoma Alismatis. methodological investigative model of quantitative analysis of four triterpenoids in Rhizoma Alismatis can be used for quantitative analysis of multi-component of Rhizoma Alismatis and be helpful for the quality control of Rhizoma Alismatis. We then to different origin Rhizoma Alismatis research on the quality evaluation, a batch of16for medicinal materials content determination. The results show that analysis of less than30min, separating degree good, peak sharp shape; Precision and repeatability and stability:RSD<210%, recovery>95%. Different origin of diarrhea contains four ze ingredient content of obvious difference, the difference in HPLC map performance characteristics. Conclusion:this method can be used for the verification of the medicinal ingredients of diarrhea ze more quantitative analysis for comprehensive evaluation ze purging quality to provide a new technology.To study the pharmacokinetic and bioavailability of23-acetyl alisol B from Rhizoma Alismatis by different administration. Method:23-acetyl alisol B was given by oral administration and intravenous injection to rats respectively and blood sample were withdrawn at different time.23-acetyl alisol B in plasma samples were determined by HPLC-UV method. The mobile phase consisted of acetonitrile and water (80:20). The detection wave length was at210nm;the flow rate was1.0mL·min-1. The model of pharmacokinetic was built by the drug-time curve and the parameters and absolute bioavailability were calculated. Compartment model analysis showed that, the pharmacokinetic model were fitted to one compartment open model after oral administration and intravenous injection in rats. The main pharmacokinetic parameters of23-Acetyl Alisol B in rats are Tmax=122.15±15.23min, Cmax=9.89±0.87(μg/mL), t1/2=58.72±6.23min, AUC0-t=1854.970±142.31(μg.min.mL-1), AUC0-∞=1875.81±144.27(μg.min.mL-1), CL/F=2.89±0.13(mL/min) for oral administration and are Tmax=10.04±0.78min, Cmax=42.59±3.47(μg/mL),t1/2=32.05±2.53min,AUC0-t=2810.08±178.70(μg.min.mL-1), AUCo-∞=2812.41±224.63(μg.min.mL-1), CL/F=1.74±0.54(mL/min) for intravenous injection. Mean bioavailability (F) is44.46%. The results show that, the absorption of23-Acetyl Alisol B in rats is slow but complete comparing with the quick elimination. It implied that23-Acetyl Alisol B has good pharmacokinetic characteristics, and can be easy to develop into a clinical drug which can be convenient to use. |
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