Study The Relationship Between The Changes Of Urinary,Serum CTGF、HGF And TVM Actual Situation Dialectical Of Chronic Renal Failure Patients

Objective:1. To investigate the relationship of the Empirical differentiation of TCM with the content of urinary and serum connective tissue growth factor (CTGF)、hepatocyte growth factor (HGF) level change on chronic kidney disease3(CKD3) and chronic kidney disease4(CKD4) patients with chronic renal failure, and the and the aplication values in chronic renal failure patients.2. To study the relationship between different indexes and.3. To evaluate the correlation between the serum CTGF、HGF and the urinary CTGF、HGF in chronic renal failure; which can reflect the lesion of renel better in the serum CTGF、HGF and the urinary CTGF、HGF.Method:Using two-layer antibody sandwich enzyme-linked immunosorbent assay (ELLSA) method determination the conttent of urinary and serum CTGF、HGF on the62chronic renal failure patients and30healthy. Collection the serum creatinine values of chronic renal failure patients, using the improved MDRD formula which Chinese eGFR collaborative group published in the JASN2006(17):2937-2944, more suitable for Chinese people to calculate the glomerular filtration rate. Study the content of urinary and serum connective tissue growth factor (CTGF)、hepatocyte growth factor (HGF) level change on CKD3or CKD4whith chronic renal failure, the relationship of the level change whith serum creatinine values (Scr), and glomerular rate filtration (eGFR). And evaluate the correlation between the serum CTGF、HGF and the urinary CTGF、HGF in chronic renal failure. Reference to the"Chronic Renal Failure Syndrome Differentiation Standard And Quantitative Classification Standard "of the"Guiding Principles of Clinical Research On New Drugs Of Traditional Chinese Medicine In Chronic Renal Failure" which editored by Zheng xiaoyu in2002, Collection the Chronic Renal Failure patients s Traditional Chinese medicine clinical symptoms,at the same time quantization. Classified The deficiency of score high classed as partial deficiency group, empirical scoring high classed as partial empirical group. study the content of urinary and serum connective tissue growth factor (CTGF)、hepatocyte growth factor (HGF) level change on the two group. Using SPSS16.0software system for calculating the measured data, The calculation results express by mean±standard deviation (X±S) Compared between groups using the Mann-Whitney U and Wilcoxon test, when p<0.05,statistically significant difference. Correlation analysis using the rank correlation analysis,when p<0.05,there has correlationship.Result:1.The content of urinary and serum CTGF、HGF in62chronic renal failure patients increased significantly compared with the Control group,a significant difference (P <0.01).2. Compared with CKD4group, the content of serum CTGF、HGF in CKD3group were no significant changes (P<0.01).3. Compared with CKD4group, the content of urinary CTGF、HGF in CKD3group were no significant changes.4. Compared with partial empirical Zheng group, the content of urinary and serum CTGF、HGF in partial deficiency Zheng group reduced significantly, the more obvious differences (P<0.05).5. There was not correlation between the urinary and serum HGF、CTGF and the Ccr、eGFR.6. There were not correlation between serum HGF and urinary HGF、the serum CTGF and urinary CTGF.Conclusion:1. The content of urinary and serum CTGF、HGF increased significantly in chronic renal failure patients. Both CTGF and HGF both possiblely took part in the pathological mechanism of renal interstitial fibrosis.2. In the CKD3period and the CKD4period of the Chronic renal failure patients, the overall level of the urinary and serum CTGF、 HGF has remained consistent, indicating that in the late chronic renal failure the content of CTGF、HGF do not increase with chronic renal progression and elevated.3. In the syndrome differentiation of TCM actual situation,compared with the partial empirical group, the content of urinary and serum CTGF、HGF levels of the the partial deficiency group were significantly decreased, indicating that in the process of chronic renal failure, with the real evil accumulation, the strengthen of the renal interstitial fibrosis increased and the secretion of CTGF and HGF increased, especially CTGF.