Study On The Difference Between Rhubarb And Stewed Rhubarb For Use

Rhubarb is the dried root and rhizome of Rheum officinale Baill. Rheum palmatum L. and Rheum tanguticum Maxim.ex Balf. polygonaceae plants. It’s a clinical traditional Chinese medicine which is frequently used as expelling pathogenic heat loosen the bowels because of its bitter taste and cold quality. Stewed rhubarb, which is made by evaporated and stewed with rhubarb, could relaxing diarrhea, easing abdominal pain and promoting blood circulation. Traditional Chinese doctors deal with rhubarb and stewed rhubarb in different ways because of different processing methods and different qualities. However, there are not too much scientific research on stewed rhubarb, and not too much scientific record about "difference between rhubarb and stewed rhubarb" and "difference between rhubarb and stewed rhubarb for use". This item is supported by the researching item "The study on normative using19Chinese medicine"(No.20110700710). We will undertake the research on rhubarb, providing theoretical basses on "difference between using rhubarb and stewed rhubarb"Systematic and overall researches on traditional processing way of rhubarb would be executed by consulting a lot of archaic medicine works and modern processing books. After arranging572relational literatures from134archaic medicine works, the method of evaporating with wine was deemed to be used at most. According to the statistics about101processing way of rhubarb from25modern processing books, stewed rhubarb was the breed which was recorded at most mow. These conclusions provide literature supports for processing rhubarb by evaporating with wine and researching stewed rhubarb in this item. In addition, we found that stewed rhubarb could relaxing diarrhea and promoting blood circulation by summarizing knowledge on principles of rhubarb processing, from archaic medicine works and modern processing theories. So literature basses would be provided for the theory that "difference between rhubarb and stewed rhubarb for use"The using situation clinically of rhubarb and stewed rhubarb would be understood from source of Chinese medicine slices, distinguish of Chinese medicine slices, promiscuous using between rhubarb and stewed rhubarb, prescribes of doctors, and the cognition of doctors and pharmaceutists to "difference between using rhubarb and stewed rhubarb", according to investigate by questionnaire to doctors or pharmaceutists of100hospitals in Sichuan province. According to the statistics, we found that rhubarb and stewed rhubarb which were used by hospitals come from non-original areas and non-GAP bases, and processing by factories mostly. Experiential distinguish wood be adopted basically before laid up. Using rate clinically of rhubarb and stewed rhubarb was50%approximately, and promiscuous using between rhubarb and stewed rhubarb existed sometimes. Though names of drags on prescribes were not standard sometimes, most doctors and pharmaceutists said yes to keep "prescribe referring". Otherwise,80%doctors and pharmaceutists knew less about traditional theories and modern researches on "difference between rhubarb and stewed rhubarb for use" of rhubarb.The best processing way of evaporating rhubarb with yellowwine would be defined, by inspecting the volume of yellowwine and water, the time for moistening, and the time for evaporating, measured by the content of total anthraquinone and free anthraquinone. The best processing way is, Rhubarb slices (2～4mm thick) or rhubarb pieces are mixed thoroughly with yellowwine and water (yellowwine:water=1:1). Moistened1.5h, and evaporated24h in food steamer separated from water, then dried in60℃.30g yellowwine and30g water will be added to every100g rhubarb. This processing method was proven that it’s stable and feasible by checking experiment and pilot study.HPLC condition and the method extracting rhubarb were defined by systematic inspecting, then the fingerprints of rhubarb and stewed rhubarb would be compared. Relative to rhubarb, the chromatographic peaks of fingerprint of stewed rhubarb changed everyone almost.12peaks declined badly or disappeared, and7peaks increased obviously (including5diagnostic free anthraquinones). So, there was superior difference with chemical constituents between rhubarb and stewed rhubarb, that chromatographic data basses would be provided for the theory that "difference between rhubarb and stewed rhubarb" It was proved that diarrhea function of stewed rhubarb was weaker than rhubarb, according to inspect the difference of diarrhea function between stewed rhubarb and rhubarb, by defecate frequentness experiment for little rats and small intestine moving experiment for little rats. This conclusion was proved by compatible research that bringing stewed rhubarb and rhubarb into Dachengqi Decoction. And it was proved that the function of promoting blood circulation of rhubarb was stronger than stewed rhubarb, according to inspect the difference of the function of promoting blood circulation between stewed rhubarb and rhubarb, according to comparing the influence for hemorheology of rats with blood stasis by high molecular dextran of stewed rhubarb and rhubarb. This conclusion was proved also by compatible research that bringing stewed rhubarb and rhubarb into Xiayuxue Decoction. Aforementioned conclusions imply that stewed rhubarb can relaxing diarrhea and promoting blood circulation to rhubarb. The pharmacological data basses would be provided for the theory that "difference between rhubarb and stewed rhubarb".Otherwise, stewed rhubarb and stewed rhubarb without yellowwine would be compared by HPLC fingerprint and hemorheology. There was no difference with HPLC fingerprint, but clear difference with hemorheology. The influence for hemorheology of rats with blood stasis by high molecular dextran of stewed rhubarb was better than rhubarb. So there is important meaning for promoting blood circulation to rhubarb that adding yellowwine in the process that the rhubarb were prepared.