Analysis Of The Characters Of RbcL/rDNA Its Sequences And The Changeable Contents Of Gallic Acid In Polyonuum Capitatum

Objective:Polygonum capitatum, diverse eco-geographic origins (wild and cultivated in Guizhou), was selected to analyzes the relationship between characters of rbcL/rDNA ITS sequence and contents of gallic acid. Thus, molecular markers developed from sequences would further be very useful for application in monitoring physiological traits and determining the sampled eco-geographic origins in Chinese traditional medicinal plants.Methods:(1) Total genomic DNA was extracted from Polygonum capitatum following a modified cetyltrimethyl ammonium bromide (CTAB) protocol.(2) The purified products of PCR amplifications were directly sequenced, associated with rbcL/rDNA ITS sequences in triplicate, using consensus primer pairs.(3) Nucleotide sequence analyses, phylogenetic tree and homology, were performed with Clustal X software and DNAMAN programme based on rbcL/rDNA ITS sequences.(4) And then, our data of rbcL/rDNA ITS sequences were submitted to NCBI to harvest GenBenk accessions.Results:(1) Just as shown in this study, high pure genomic DNA was validated to perform PCR reaction based on the values of OD260/OD280ratio (from1.1to2.0).(2) The sequencing analysis results indicated that the sequences of rbcL were from979to985bp in Polygonum capitatum, as compared with variable rDNA ITS sequences (661-666bp).(3) High homology was observed by sequence blast and clusters of rbcL/rDNA ITS in Polygonum capitatum.(4) GenBank accessions were obtained by submissions of rbcL/rDNA ITS sequences in NCBI.Conclusion:Overall, we argued that rbcL/rDNA ITS sequences could be applied to explore molecular identification to determine the sampled eco-geographic origins in Polygonum capitatum. Objective:to determine the contents of different class gallic acid content, to explore molecular identification to determine the sampled eco-geographic origins in Polygonum capitatum.Methods:using high performance liquid chromatography, DiamonsilC18(4.6mm×250mm,5μm) for chromatographic column; Mobile phase for methanol-0.2%phosphoric acid (45:55), velocity of1.0ml/min, detected wavelength350nm.Results:contents of different class contained in the gallic acid content of obvious differences in (0.22-0.36) between.Conclusion:the method is scientific and accurate, simple, control, and evaluation of origin for different class provides the basis for the quality of the contents.