Nanog Is Highly Expressed In Esophageal Cancer And Its Downregulation By RNAI Make Esophageal Carcinoma Cell Lines Proliferation Slow Down

ObjectiveEsophageal is one of the most common malignancies, now of the maintreatment is surgical treatment, but5year’s survival rates still not optimistic. Thecancer stem cell theory in recent years tumors, development, relapse andmetastasis and treatment to bring new ideas and new ideas. We want to passon stem cell transcription factors in patients with esophageal Nanog expressingthe meaning of research, to further screening esophageal pathologyclassification of clinical diagnosis, treatment and provide theoretical foundationand experimental basis.Method1, Through the immunohistochemical detection Nanog protein expression inpatients with esophageal organization and meaning;2, Through the cellular immune fluorescence detection esophageal TE-1celllines, Eca-109Nanog protein expression and meaning;3, Application LipofectAMINE2000fluorescent markers will be the NanogsiRNA into cells, through the fluorescence microscope RNAi efficiency, againthrough the RT-PCR and Western Blot test siRNA silence efficiency;4, By MTT method after the group of cells RNAi measured96h growth change. Results1, Nanog in patients with esophageal cancer than in adjacent tissues obviouslyhigh expression, mainly in the expression in the cell plasma, and the patient’sgender, age and clinical characteristics irrelevant; And the patient’s installmentdirect proportion, and inversely proportional to differentiation degree, and thestatistical significance;2, Nanog protein in patients with esophageal cell lines TE-1, Eca-109highexpression, the main role in the nucleus, cell plasma also have expressed,expression to have differences between the cells;3, Cell RNAi rate is （67.57±16.90)%, Nanog siRNA silence efficiency can beas high as80%above;4, turn and then measured96hours Nanog siRNA group is the control groupcell proliferation have slowed, and the statistical significance.Conclusions1, In patients with esophageal tissues, Nanog amount of protein expression washigher than the cancer organizations, and esophageal pathology classificationand the with differentiation degree involved; In patients with esophageal Nanogthe occurrence, development and maintaining cell differentiation of low plays animportant role, may be induced esophageal matured ideal targets, theevaluation of esophageal malignant to evaluate the degree of a certain value.2, Nanog protein in patients with esophageal cell lines Eca109and TE-1highexpression, the expression of the differences between the cells that celldifferentiation of different state, hints of the existence of the cancer stem celltreatment; Through the RNAi technology to reduce its expression of the value ofthe cells that slows down, that reduce Nanog protein expression to slow thegrowth of esophageal value-added, tip by inhibiting Nanog expression may beone of the ways of esophageal treatment.