Effects Of Ethanol On Male Mice Fertility

Objective：Discussion the effects of ethanol on male mice fertility, indirectlyreflect the impact of alcohol on male fertility,for the prevention and treatment ofmale infertility and provide scientific basis.Methods:Trials selection7-12week old male mice50healthy, weight25g~30g,were randomly divided into five groups, each10, respectively, for the control group,the experimental group (Group4).Ethanol were fed on a daily basis the amount ofthe test group is divided into0.1ml,0.2ml,0.3ml,0.4ml four groups.Mice were fedonce a day after pre-fed one week. Trials in mice fed the4th,8th,12th day slicingproduction, HE staining, germ cell apoptosis was detected by gel electrophoresis,TUNEL assay for quantification of germ cell apoptosis and Bc1-2/Baximmunohistochemistry tests.Results⑴0.1ml/kg group and control group structure of Mice Testis Tissue developmentare normal, and the seminiferous epithelium intact.Group0.2ml/kg seminiferoustubule epithelial cell layers was no significant reduction in primary spermatogeniccells and secondary spermatogenic cells loose structure, but the basal instinctspermatogenic remain intact.Group0.3ml/kg seminiferous tubule epithelial celllayers of less primary spermatogenic cells and secondary spermatogenic cellstructure is destroyed, the extreme reduction of the number of spermatogonia,irregular nuclear morphology.Group0.4ml/kg testicular tissue structure has beenseverely damaged, the seminiferous tubules of the seminiferous epithelium from thebase film off, separated from the connective tissue, of primary spermatogenic cells,and secondary spermatogenic cell structure is completely destroyed, epithelial cellsstratification can not be identified.The spermatogonia disappear secondaryspermatocytes and sperm cell nucleus volume was significantly reduced andirregular, no sperm in the lumen. ⑵Apoptosis of spermatogenic cells of the testis tissue DNA characteristic ladderwas detected in the4d and8d, the control group, group0.1ml/kg, group0.2ml/kg,0.3ml/kg group,0.4ml/kg group were not See apoptosis characteristic ladder; section12d, the control group and0.1ml/kg group does not detect a characteristic ladder, thesolvent group detected characteristic ladder.⑶TUNEL assay for quantification of spermatogenic cell apoptosis on day4,group0.3ml/kg, spermatogenic cell apoptosis index of0.4ml/kg group comparedwith the control difference was significant (P <0.05); day80.2ml/kg groupspermatogenic cell apoptosis index and0.3ml/kg group than the control groupdifference was significantly (P <0.05), spermatogenic cell apoptosis index of0.4ml/kg group than the normal group and negative group difference is extremelysignificant (P <0.01);12days, group0.3ml/kg,0.4ml/kg group than in the normalgroup spermatogenic cell apoptosis index was significantly different (P <0.05),which8days0.4ml/kg of experimental data obtained up to0.4, the maximum valuefor the experimental data.⑷E thanol male mice spermatogenic cells apoptosis genes Bax and Bcl-2proteinexpression, the positive area increases as the ethanol concentration increased in adose-response relationship, with increasing ethanol concentration gray valuedecreases. With the increase in the concentration of ethanol positive area than thelower ethanol concentrations gray values than the control group, but difference wasnot significant (P>0.05), description of the concentration of high doses of ethanolcauses the Bcl-2protein expression reduced.In view of these results, sustained high doses of alcohol can induce proapoptoticgene Bax protein expression and prevent inhibition of apoptosis protein Bcl-2geneexpression; Sustained high doses of alcohol can induce testicular germ cell apoptosis,which damage the reproductive capacity in mice.