Effects Of Aspirin On VEGF, β-catenin Expressions In Colon Cancer Cell Lines Of HT-29and SW480Cells

Objective:To Investigate the aspirin inhibiting mechanism on growth and Proliferation of cultured human colon caner celles line HT-29and SW480,whicn to provide the experimental and theoretical basis for clinical treatment and research of colon cancer.Methods:Applying aspirin on cultured human colon cancer celles line HT-29and SW480, Using3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromid (MTT) colorimetric assay for detection of growth and proliferation of HT-29and SW480cells,real-time quantitativereverse transcripton polymerase chain reaction(Real-time PCR) on detection of β-catenin and vascular endothelial growth factor(VEGF) mRNA’s expressions, protein blotting(Western-blot) on detection of β-catenin and VEGF protein’s expressions.Results:Aspirin on cultured human colon HT-29and SW480cell, Inhibition rates were increased gradually with the increase of the drug concentration, there was a dose-response relationship; At the same time, as the extension of times, the inhibition rates were increased gradually too, had a time-effect relationship. The expression level of mRNA and protein, VEGF,β-catenin’s mRNA and protein expression level were reduced with the same concentration of aspirin on cultured human colon HT-29, except6,12,24and48hours intervention group compared with the control have differences significantly (p<0.05,respectively), which exist a time-effect relationship. At the same time, VEGF,β-catenin’s mRNA and protein expression level also reduced with the same concentration of aspirin on cultured human colon SW480, except6,12,24and48hours intervention group compared with the control also have differences significantly (p<0.05, respectively) and a time-effect relationship too.Conclusions:Aspirin on growth and proliferation of cultured human colon cancer cells line HT-29and SW480had significantly inhibitory effects, it has a time-and dose-dependent relationship. The expression of the VEGF and β-catenin on mRNA and protein on cultured human colon celles line HT-29, SW480were reduce by aspirin, which may be one of the mechanism of aspirin’s anti-cancer effect; At the same time, may be there exists a certain correlation between VEGF and β-catenin; Aspirin as an inhibitor of Wnt signaling pathway, it may control beta-catenin expression and VEGF expression to inhibit tumor growth, but its action mechanism need further studying.