Pharmacological Activity Research Of Inula Britannica Flower Polysaccharide And Flavonoids

Purpose：Research Inula britannica flower polysaccharide(IBP) and Inula britannicaflower flavonoids (IBFF) hepato-protective activity, as well as IBP antidiabetic activity.Methods：1. Protective effect of IBP and IBFF on acute liver injury in mice.(1) Using CCL4and Bacillus Calmette Guerin (BCG) and lipopolysaccharide (LPS)induce liver injury model in mice.Detect the levels of AST and ALT in the serum;Detect the contents of SOD、MDA and GSH-PX in the liver tissues.(2) Using D-Gal induce the acute liver injury model in mice;Detect the levels of AST, ALT in serum;Detect the contents of SOD, MDA, GSH-PX and CAT in the liver tissue;Detect the content of TNF-α, COX-2and iNOS in serum;Pathological analysis of liver tissue;Detect the expression of TNF-α, COX-2and iNOS mRNA in liver tissue of mice.2. Protective effect of IBP on diabetes in mice.Using alloxan induce diabetes model in mice. Detect the levels of weight, fasting bloodglucose, liver glycogen, cholesterol and triglyceride, LDL-C and HDL-C levels in mice.Results：1. Protective effect of IBP and IBFF on acute liver injury in mice.(1) IBP (50,100mg/kg) can significantly reduce transaminase and MDA levels on acuteliver injury induced by CCL4and BCG plus LPS, and it can increase the levels of SODin liver tissue of mice(p<0.05, p<0.01).(2) IBFF (250,500mg/kg) can significantly reduce the elevated of AST and ALTinduced by D-Gal (p<0.05, p<0.01);IBFF (250,500mg/kg) can significantly reduce the elevated of MDA, and significantly increase the content of SOD, GSH-PX, CAT in liver injury mice (p<0.05, p<0.01).IBFF (250,500mg/kg) can significantly reduce the content of TNF-α, COX-2and iNOSin liver injury mice induced by D-Gal (p<0.05, p<0.01);IBFF (250,500mg/kg) can significantly reduce the liver tissue necrosis, inflammatorycell infiltration, lose of cytoplasm, cell swelling and fatty degeneration of mice in liverinjury caused by D-Gal (p<0.05, p<0.01);IBFF (250,500mg/kg) can significantly reduce the content of inflammatory factor withTNF-α, COX-2and iNOS mRNA in liver injury mice induced by D-Gal (p<0.05,p<0.01).2. Protective effect of IBP on diabetes in mice induced by alloxan.IBP (50,100mg/kg) can significantly resist the reducing of body weight, decreasedblood glucose levels and TC/TG ratio, decreased the levels of LDL-C and increase thelevels of HDL-C in serum of diabetic mice (p<0.05, p<0.01).Conclusion:1. IBP and IBFF can significantly enhance the ability of antioxidant and reduce lipidoxidation injury in liver tissue induced by CCL4and BCG plus LPS;IBFF can inhibit hepatitis injury because of reducing the content of TNF-α, COX-2,iNOS of liver tissue in mice.2. IBP can significantly resist reduce of the body weight, increase glucose levels anddecrease TC/TG ratio, decreased the levels of LDL-C and increase the levels of HDL-Cin serum of diabetic mice (p<0.05, p<0.01) in serum in diabetic mice induced by alloxan.