Studies On Chemical Constituents Of Persistent Calyx Of Physalis Pubescens L.

Physalis pubescens L.，an annual or perennial herbage, belongs to Physalis Genus,Solanaceae, which is mainly distributed in the three provinces in Northeast China andInner Mongolia, and grows in meadows or wayside. The fruits of Physalis pubescens tastesucculency, sweet with a little sour, and won great favor among the Asian people. In China,the fruit and the whole plant can also be used as herb medicine, and has the effects ofdetoxification and anti-inflammation in the traditional Chinese medicine. Currently, theextracts of fruits of the Physalis genus have been reported to have potent antioxidant,anti-inflammatory, and cytotoxic activities. But in the northeast region of China, Physalispubeacens L. is largely planted by some farmers, and instead of herb medicine, the berriesare mainly sold as delicious fruits or raw materials for producing juice, jams and wine.This plant has also been grown in America, especially in the southern half of the UnitedStates. Berries have been shown to provide significant health benefits, because of their highantioxidants, vitamins, minerals and fiber. Previous studies on the Physalis genus have ledto the isolation of flavonoids, steroids, alkaloids and many kinds of fat-soluble oilcompositions, such as aldehydes, esters, organic acids and so on.During the course of investigation for bioactive agents from persistent calyx ofPhysalis pubescens L.,48compounds were isolated, and28compounds were identified tobe3,6,11-trimethyl-3-hydroxyl-1,6E,10-dodecatriene-8-O-β-D-glucoside（ME-1）,Philadelphicalactones A（ME-2）, Virginols A1（ME-3）, physalis pubescensol A (ME-4),β-sitosterol(ME-5),daucosterol(ME-6),3,7,4′-trimethyl-myricetin(ME-7),quercetin(ME-8),Kaempferol(ME-9),3′,5-dihydroxy-3,7,4′-trimethoxylflavone(ME-10),3,3′,4′,5-tetrahydroxy-7-methoxyflavone(ME-11),kaempferol-3-O-β-D-sophoroside(ME-12),kaempferol-3-O-α-L-rhamnosterin-(1→6)-β-D-glucoside(ME-13),kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnosterin(ME-14),kaempferol-7-O-α-L-rhamnopyranoside(ME-15),kaempferol-3-O-α-L-rhamnopyranoside(ME-16),kaempferol-7-O-β-D-glucoside-3-O-β-D-glucoside(1→2)-β-D-glucoside(ME-17),kaempferol-3-O-β-D–galactopyranoside(ME-18),kaempferol-3-O- β-D-glucoside(ME-19),genistein-7-O-β-D-gIucopyranoside-4′-O-α-L-rhamnopyranosy-(1→2)-β-D-glucopyranoside(ME-20),quercetin-3-O-β-D-glucoside(ME-21),quercetin-3-O-β-D-galactopyranoside(ME-22),quercetin-3-O-β-D-arabinopyranoside(ME-23), quercetin-3-O-α-L-rhamnoside (ME-24), rutin (ME-25), Ginkgolide A (ME-26), Ginkgolide B(ME-27),3-hydroxyl-4-methoxy benzoic acid (ME-28). Among them, compound1and3are new compounds, and named as Physaliside A, Virginols A1,compounds10~20,22~28were isolated from Physalis Genus for the first time, and compounds2was isolatedfrom the plant for the first time.In this paper, we report the isolation and structural elucidation of these compounds.