The Association Research Of The Rs7789045Gene Polymorphism Of NOD1in A Chinese Population With Gastric Cancer

BackgroundGastric cancer is one of the most common malignant human cancers worldwide, accounting for the second mortality of various maliganancy. In recent years, the incidence rate of gastric cancer showed a gradual upward trend. China is one of the countries with the highest incidence of gastric cancer and mortality in the world. Most of the study have shown that gastric cancer and Helicobacter pylori(H.pylori) have the similar epidemiological characteristics. The incidence of gastric cancer is closely related to the inflammation process of host body caused by the H.pylori infection on human. Nucleotide-binding oligomerization domain (NOD) family of proteins is one of the pattern recognition receptors of the host pathogen recognition, and research has proved the NOD1gene polymorphism and clinical outcomes caused by H.pylori infection is relevant. Although studies have shown that the NOD1E266K(rs2075820) mutation increased the risk of antral atrophy, peptic ulceration and intestinal metaplasia, there is little research related to the association between NOD1polymorphisms and gastric cancer.ObjectiveTo provide the basis for early diagnosis and prevention of gastric cancer, this study focused on the relationship between the NOD1(rs7789045) polymorphism and the risk of gastric cancer.MethodsWe took a case-control study, including a sample of503patients with gastric cancer as the case group,299patients with gastritis samples and226healthy sample as a control group. Serum levels of anti-H.pylori IgG were measured by enzyme-linked immunosorbent assay to indicate H.pylori infection. The single nucleotide polymorphism (SNP) in the NOD1(rs7789045) was analysised by the Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), and the results were statistically analyzed.Results1、We had the serum samples from160patients of gastritis and296patients of gastric cancer in all populations, which was detected on H.pylori infection by ELISA The H.pylori infection rate is74.7%in gastric cancer patients compared to74.4%in gastritis patients. The χ2test proved that the result was not statistically significant(P=0.946,>0.05), which indicated that H.pylori infection was not significantly different berween gastritis and gastric cancer patients.2、503cases of gastric cancer patients were compared with299gastritis group and226healthy group, and we found that compared to carriers of NOD1(rs7789045) AA genotype, the risk of gastric cancer increases in TT genotype carriers (P=0.039, OR=1.564,95%CI:1.024-2.388)、(P=0.041, OR=1.633,95%CI:1.021-2.613)3、The distribution of TT and AA genotype was signigicantly different between H. pylori-positive gastric cancer and gastritis patients, and OR value (OR=2.139,95%CI:1.197-3.822) increased compared to all patients with gastritis and gastric cancer without the factors of H.pylori infection, which indicated that NOD1(rs7789045) TT genotype increased the risk of H.pylori associated gastric cancer.Conclusions1、There was no significant difference in H.pylori infection rates between gastric cancer and gastritis patients.2. NOD1(rs7789045) gene polymorphism was related to gastric cancer, and NOD1(rs7789045) TT genotype may be the suspective factors of gastric cancer.3. NOD1(rs7789045) gene polymorphism may play an important role in pathogenesis of gastric cancer with H.pylori infection and thus affect H.pylori infection risk for gastric cancer.