Association Study Of CD36Single Nucleotide Polymorphisms With Atherosclerotic Cerebral Infarction In Elderly Patients

BACKGROUNDCerebral infarction is one of the three diseases that cause human death currently. Each year about4.6million people died of cerebral infarction in the world and approximately50%to70%people who survival in cerebral infarction left with severe disability, bringing heavy burden to the society and family. The pathogenesis of cerebral infarction is complex, and that is the result of the interaction of genetic and environmental factors. Genetic factors play an important role in the pathogenesis of cerebral infarction. In recent years, people found that there were closely relationship between CD36and risk factors of cerebral infarction, such as atherosclerosis, type2diabetes, dyslipidemia, etc. According to a genome-wide association study (GWAS) showed that15CD36SNPs can increase the risk of stroke. However, correlation research about CD36SNPs and cerebral infarction has not been reported.OBJECTIVEAccording to study CD36SNPs in genotype distribution of han population in Shandong district, explored the relationship between CD36single nucleotide polymorphisms and atherosclerotic cerebral infarction in elderly patients and search for genetic risk factors for cerebral infarction. METHODSIn the case-control study,121patients with atherosclerotic cerebral infarction which were older than75years were enrolled into the case group and217age-and sex-matched healthy examined people without cerebral infarction as the control group. Height, weight, body mass index and blood pressure were measured. All subjects completed a questionnaire about cerebrovascular disease risk factors on present condition, such as history of hypertension, coronary artery disease and diabetes. Furthermore, fasting blood sample was collected from each subject afterl2-14hours fast to determine blood cell analysis and fasting blood glucose(FBG), triglycerides(TG), total cholesterol(TC), low-density lipoprotein(LDL), high-density lipoprotein(HDL). Enzyme linked immunosorbent assay (ELISA) was used for determination of plasma thromboxane B2(TXB2). Using polymerase chain reaction-ligase detection reaction (PCR-LDR) detected the genotype of rs1761667and rs9784998which are two locis of CD36and analysis the correlation of CD36SNPs and elderly atherosclerotic cerebral infarction (ACI).RESULTS1. The case group body mass index, blood pressure, blood sugar, triglyceride, total cholesterol, low-density lipoprotein, thromboxane B2higher than the control group, high density lipoprotein significantly lower than the control group, there was significant difference between the two groups (P<0.05).2. There were no significant differences in genotype and allele frequencies of rs9784998between case and control group (P>0.05).3. The rs1761667genotype AG frequency distribution was significantly higher in the patient group, with a statistically significant difference between the two groups (P <0.01), while allele distribution was no significant difference (P>0.05).4. Logistic regression analysis and stepwise regression analysis showed that rs1761667AG genotype can increase the risk of cerebral infarction (OR=1.93,95%CI=1.08-3.45, P<0.05; OR=1.88,95%CI=1.06-3.34, P<0.05).5. Thromboxane B2levels of the case group was significantly higher than the control group, with a statistically significant difference (P<0.01), but the AG genotype crowd thromboxane B2levels between the two groups was no significant difference (P>0.05).CONCLUSION1. rs1761667SNPs are statistically associated with atherosclerotic cerebral infarction.2. rs1761667genotype AG is a genetic risk factor for atherosclerosis cerebral infarction.3. rs9784998SNPs is not associated with atherosclerotic cerebral infarction in statistically.4. CD36gene polymorphisms are not correlated with thromboxane B2.