Association Of CD40Gene(-1C/T) Polymorphism With Ischemic Cerebrovascular Disease

Objective To investigate whether CD40gene (-1C/T) single nucleotide polymorphism(SNP) is associated with the stability of carotid artery plaque, while analyse the association CD40gene (-1C/T) single nucleotide polymorphism(SNP) and occurrence of ischemic cerebrovascular disease..Methods:170patients with acute ischemic cerebrovascular disease were selected as cerebral infarction group,all subjects were detected the plaque echogenicity and morphology were evaluated bilaterally by carotid ultrasonography. According to the results, all patients in cerebral infarction group were divided into three subgroups, including unstable plaque group(51cases), stable plaque group(60cases) and non-plaque group(59cases)and61persons without cerebrovascular disease were selected as control group. The gene polymorphism was measured by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFL P).Results:①The frequency of the C allele (52.9%) and the CC genotype (30.6%) in cerebral infarction group was significantly higher than that of the controls (38.5%,16.4%).(X2=7.466, P=0.006; X2=4.606, P=0.032). The frequency of the T allele (47.1%) and the TT genotype (24.7%) in cerebral infarction group was significantly lower than that of the controls (61.5%,39.3%).(X2=7.466, P=0.006; X2=4.714, P=0.030).②The frequency of the C allele (75.5%) and the CC genotype (52.9%) in unstable plaque group were significantly higher than that in stable plaque group (53.3%,30%), and were both significantly higher than that in non-plaque group63.1%11.9%).(stable plaque group vs non-plaque group:X2=9.970, P=0.002; unstable plaque group vs stable plaque group:X2=11.680, P=0.001; unstable plaque group vs non-plaque group:X2=39.532, P=0.000. stable plaque group vs non-plaque group:X2=5.896, P=0.015; unstable plaque group vs stable plaque group:X2=6.019, P=0.014; unstable plaque group vs non-plaque group:X2=21.613, P=0.000.) The frequency of the T allele (24.5%) and the TT genotype (2%) in unstable plaque group were significantly lower than that in stable plaque group (46.7%,23.3%), and were both significantly lower than that in non-plaque group (66.9%,45.8%).(stable plaque group vs non-plaque group:X2=9.970, P=0.002; unstable plaque group vs stable plaque group:X2=11.680, P=0.001; unstable plaque group vs non-plaque group:X2=39.532, P=0.000. stable plaque group vs non-plaque group:X2=6.627, P=0.010; unstable plaque group vs stable plaque group:X2=10.774, P=0.001; unstable plaque group vs non-plaque group:X2=27.659, P=0.000.)③The C allele increased the risk of cerebral infarction as the cerebral infarction group compared with the the controls.(OR=1.795,95%CI1.177-2.738).④The C allele increased the risk of the formation of atherosclerotic plaque as the stable plaque group compared with the controls.(OR=2.315,95%CI1.369-3.914), the C allele increased the risk of the formation of unstable plaque as the unstable plaque group compared with the stable plaque group.(OR=2.695,95%CI1.514-4.796), the C allele increased the risk of the formation of unstable plaque as the unstable plaque group compared with non-plaque group.(OR=6.239,95%CI3.451-11.280)Conclusions:CD40-1C/T polymorphism is associated with the development of the formation of carotid atherosclerosis plaque, the formation of unstable stable carotid atherosclerosis plaque,and ischemic cerebrovascular disease.The C allele frequency maybe participate the development of the formation of carotid atherosclerosis plaque and stable carotid atherosclerosis plaque to unstable by promoting plaque rupture, which can lead to occurrence and recurrence of ischemic cerebrovascular disease.