The Change Of Blood Pigment Epithelium-derived Factor Level And Platelet Activation,Platelet Aggregation In Hepatitis B Cirrhosis Patients

Objective: To detect the change of CD62P、CD63、PAgT and PEDF level inhepatitis B cirrhosis patients and normal control group， to study its correlationrelationship between PEDF level and platelet activation， platelet aggregation inpatients with hepatitis B cirrhosis.Methods: According to Child-Pugh classification standard，hepatitis B cirrhosispatients could be divided into group A、group B and group C，compared to normalpeople，using flow cytometry to detect CD62P、CD63positive percentage，plateletaggregation analyzer to detect PAgT， and biotin double antibody sandwichenzyme-linked immunosorbent method to detect the contents of PEDF.Results：（1）In hepatitis B cirrhosis patients blood，group A、group B and groupC CD62P positive percentage（13.25±4.87、12.26±1.37、9.81±2.42）was significantlyhigher than normal control group （4.89±1.80）， the difference was statisticallysignificant (P <0.05);compared with group A，group C positive expression of CD62Pwas decreased obviously，the difference was statistically significant (P <0.05).（2）In hepatitis B cirrhosis patients blood，group A、group B and group C CD63Positivepercentage（2.69±1.27、2.99±1.85、2.49±1.61）was not different from the normalcontrol group（2.31±1.22）（F＝0.291，P＞0.05）（.3）In hepatitis B cirrhosis patientsblood，group A、group B、group C platelet PAgT（17.37±5.85、27.14±4.57、17.14±7.93）was significantly lower than the normal control group（37.26±8.75），thedifference was statistically significant (P<0.05);group A and group C PAgT wasdeclined significantly compared with group B (P <0.05).（4）Serum concentration ofPEDF（50.87±5.70、44.11±5.28、49.52±5.70）in hepatitis B cirrhosis patients was decreased significantly compared with the normal control group（233.40±14.11），difference was statistically significant (P <0.05);but there was no difference in thecomparison between groups in terms of liver cirrhosis (P>0.05).（5）In hepatitis Bcirrhosis patients blood，PEDF expression was negatively related with the CD62P (r＝-0.578，P <0.05)；PEDF expression was no related with CD63(r＝-0.333，P>0.05); PEDF expression was positively related with PAgT (r＝0.505，P <0.05).Conclusions: In hepatitis B cirrhosis patients blood，protective factors PEDFexpression reduced，platelets were abnormally activated，platelet aggregation functiondroped； PEDF in hepatitis B cirrhosis patients blood could influence plateletactivation and platelet aggregation；May partly explain why the cirrhosis of the liverbleeding risk increases.