| Objective: Intracerebral hemorrhage as a serious cerebrovascular event,Ithave a high incidence.and have been harm to human health seriously. Inaddition to the mass effect of the hematoma after intracerebralhemorrhage,there are also subsequent Brain damage around the hematoma,Such as intracerebral ischemia, intracerebral edema, inflammation, increasedintracranial pressure and so on. In recent years, brain edema and inflammatoryresponse have become hot topic. Aquaporin-4(AQP-4) is a Key channel forwater flow in the brain. It’s a indicator of intracerebral edema. Tumor NecrosisFactor(TNF-α) is an inflammatory factor. Interleukin-10(IL-10) is animportant anti-inflammatory cytokines.They can react inflammatory responsein some ways. Dexamethasone is a glucocorticoid. It have been used for a longtime for the function of anti-inflammatory, immunosuppression, anti-shock.Intravenous route of administration. Veins are mainly route of administration.But in addition to the above roles. Dexamethasone can cause gastrointestinalulcers, osteoporosis, high blood pressure and other adverse reactions.Intravenous route of administration is more apparent. Topical route ofadministration is safer. The purpose of this experiment is to explore thechanges of AQP-4, TNF-α and IL-10after injecting dexamethasone into thehematoma cavity of intracerebral hemorrhage in rats. Providing the basis forintracranial hematoma aspiration combine with dexamethasone in clinical.Methods:108healthy male SD rats which weight are between250g and300g, are randomly divided into three groups: Sham-operated group,intracerebral hemorrhage model group and dexamethasone treatment group.There are36rats in each group. Among these18rats are for the determinationof brain water content. Fixing the rats on the stereotactic instrument after the rat were anaesthetized. Injecting non-heparinized autologous arterial bloodinto the right caudate nucleus to Preparing for rat model of intracerebralhemorrhage. The intracerebral hemorrhage model group and dexamethasonetreatment group are injected50μl autologous arterial blood. TheSham-operated group is injected nothing. Selecting qualified rats according tothe Longa standard after24hours. The Sham-operated group and intracerebralhemorrhage model group are injected saline at the same site in the hematomacavity according to0.2ml/kg. The dexamethasone treatment group are injecteddexamethasone according to1ml/kg. Choose six rats at48hours,3days and7days Respectively. Take hematoma surrounding brain tissue and then cut into6slices to observe AQP-4, TNF-α and IL-10positive cells byimmunohistochemical methods. Take five high-power field in Each section.Calculate the percentage and the average of the number of positive cells perhigh power field. Cut off the head of six rats and get the brain at three timepoints,and then measure wet weight and dry weight. Calculate the watercontent of the brain tissue. And then use SPSS13.0statistical data to observethe alteration of each indicator at different phase from the hematomasurrounding brain tissue.Results:1The Changes of the Water Content of the Rat Brain Tissue:The brain water content of the intracerebral hemorrhage group and thedexamethasone treatment group both increase at48hour, reach the peak onthe third day after intracerebral hemorrhage and decrease on the seventh day.The water content of the intracerebral hemorrhage group increase comparedwith the Sham-operated group on the third day. It also increase compared withthe dexamethasone treatment group on the third day, have a significantdifference (P<0.05). The two groups have no statistically significantdifferences at the48hour and7day.2The Changes of the AQP-4Expression:The intracerebral hemorrhage group compare with the sham-operatedgroup, the percentage of AQP-4positive cells increase at48hour,3day and7 day after intracerebral hemorrhage, have a statistically significant difference(P<0.05). It increase at48hour,reach the peak on the third day and decline onthe seventh day, the dexamethasone treatment group also has such a law.Compare with the dexamethasone treatment group，the percentage of AQP-4positive cells of the intracerebral hemorrhage group increase on the third day,P<0.05, have a statistically significant difference. The two groups have nostatistically significant differences at the48hour and7day.3The Changes of the TNF-α Expression:Compare with the sham-operated group, the percentage of TNF-αpositive cells of the intracerebral hemorrhage group and the dexamethasonetreatment group all increase at48hour,3day and7day after intracerebralhemorrhage. have a statistically significant difference (P<0.05). Theexpression of TNF-α begin to increase in48hours, peak on the third day anddecline on the seventh day after intracerebral hemorrhage. And intracerebralhemorrhage group compare with the dexamethasone treatment group, theexpression of the three time points are all higher P<0.05, have a statisticallysignificant difference.4The Changes of the IL-10Expression:Compare with the sham-operated group, the percentage of IL-10positivecells of the intracerebral hemorrhage group and the dexamethasone treatmentgroup all increase at48hour,3day and7day after intracerebral hemorrhage.Both have a statistically significant difference (P<0.05). The expression ofIL-10begin to increase at48hour, peak on the third day and decline on theseventh day after intracerebral hemorrhage. And the intracerebral hemorrhagegroup compare with the dexamethasone treatment group, the expression of thethree time points are all lower. P<0.05, have a statistically significantdifference.Conclusions:1The method of rat autologous arterial blood injection produceintracerebral hemorrhage model have higher successful rate.2The expression of AQP-4and water content around the hematoma have a positive relationship.3The high expression of TNF-α after intracerebral hemorrhageparticipates in brain edema formation.4Dexamethasone is injected into the hematoma cavity can reduce thecontent of intracerebral edema. The expression of TNF-α reduce but theexpression of IL-10increase Around the hematoma, it show thatdexamethasone can can reduce intracerebral edema, restrain the expression ofTNF-α, enhance the expression of IL-10. Ease further injury of the nervefunction after intracerebral hemorrhage. Providing the experimental evidencefor intracranial hematoma aspiration combine with dexamethasone. |
PDF Full Text Request