Study On Anti-Tumor Activity And Chemical Constituents Of Artemisia Sacrorum Ledeb.

Artemisia sacrorum Ledeb. has been used as ethnic medicines for the treatment of hepatitis by koreans for a long time. In recent years, some people have been studing on the pharmacological activity of Artemisia sacrorum Ledeb. and have achieved some results. But there is no research on systematic anti-tumor effects of Artemisia sacrorum Ledeb. and its effective components. Artemisia sacrorum Ledeb. and Artemisia annua both are Artemisia plants, and maybe contains a number of structurally similar chemical compositions, such as coumarins, terpenoids and flavonoids, etc. Therefore, it is necessary to screen the anti-tumor activity of Artemisia sacrorum Ledeb. and study its chemical constituents. It is reported that Artemisia sacrorum Ledeb. extract showed cytotoxic activity against cancer cells. This shows that Artemisia sacrorum Ledeb. contains the similar constituents that found in Artemisia annua or unique components that is effective on anti-tumor activity. Thus, this study is carried out on anti-tumor activity and chemical constituents of Artemisia sacrorum ledeb.In this research, Artemisia sacrorum Ledeb. was extracted by 95% ethanol, and then was suspended in water and successively partitioned and extracted with petroleum ether, ethyl acetate and n-butanol. The ethanol extract and other extracts were screened for anti-tumor activity. And the active sites of the ethyl acetate and n-butanol fraction were separated by different chromatographic methods (such as Silica gel column, macroporous adsorbing resin, ODS, Sephadex, Prep-HPLC, atc.). Twenty seven compounds were isolated from Artemisia sacrorum Ledeb., and their structures were elucidated by their physical and chemical properties and NMR techniques(such as1-NMR,13C-NMR, DEPT, HMBC, HSQC,1H-1HCOSY, NOESY, atc.). Their structures were identified as lignoceric acid (1), n-Octadecane (2),42 alkyl acid (3), Decane (4), Dodecane (5),β-sitosterol (6),4-hydroxy-acetophenone (8), isoscopoletin (9),5-hydroxy-7,4’-dimethoxy flavones (10), genkwanin (11), acacetin (12),7,3’,4’-Trimethylquercetin (13), scopoletin (15), coumarin (16), Santamarine (17), chrysoeriol (18), diosmetin (19), hispidulin (20), apigenin (21), scopolin(22),2-hydroxy-6-methoxy-acetophenone-4-O-β-D-glucoside (23),6-demethoxy-4’-methoxycapillarisin-7-O-β-D-glucoside (24),6-methoxy-quercetagetin-7-O-β-D-glucoside (25),3a,16a-dihydroxykaurane-17-O-β-D-gluco- side (26),16a-hydroxykaur-3-one-17-O-β-D-glucoside (27), The structures of compounds 7 and 14 to be determined. To the best of our knowledge, compounds 13、17 and 25 were isolated from the Artemisia species for the first time, and compounds 2、3、10、13、17、18、19、20、24 and 25 were isolated from the plant for the first time.