The Structure Of The Tea Polysaccharide Source Identification, Chemical Modification And Structure-activity Relationship Of The Discussion

This thesis main focused on the polysaccharide whose are teapolysaccharide, tea flower polysaccharide and tea seedpolysaccharide chemical structure, advanced chemical structure ofpolysaccharide chemically modified, and a simple structure-activityrelationship study. For use of the source of tea polysaccharides haveimportant scientific significance, and can promote the various parts ofthe tea can make use of the resources are fully utilized to create agood economic and social benefits. The research topics include thefollowing sections:(1) The use of the methylation analysis of polysaccharides the teasource polysaccharide NTPS, ATPS2, TFPS1TFPS2-2, NTSPS1residue typeanalysis. Experimental studies have shown that: contains10kinds of teapolysaccharide neutral sugar NTPS residues, respectively:�'3,6-D-Manp1�'、T-D-Manp1�'、�'5-L-Araf1�'、�'2-L-Arap1�'、T-D-Glup1�'、�'4,6-D-Glup1�'、�'6-D-Glup1�'、T-D-Galp1�'、�'6-D-Galp1�'and�'3-D-Galp1�'.The�'6-D-Glup1�'in the percentage of cases amaximum of26.90%. Tea polysaccharide acidic sugar ATPS2containseight kinds of residues, respectively, as follows:�'2,4-L-Rhap1�'、�'2-L-Fucp1�'、�'5-L-Araf1�'、�'6-D-Glup1�'、T-D-Galp1�'、�'3-D-Galp1�'、�'6-D-Galp1�'and�'4-D-GalpA1�'.The�'4-D-GalpA1�'in thepercentage of cases a maximum of49.10%. Tea flower neutralpolysaccharide sugar TFPS1contains10kinds of residues, respectively,as follows:�'3,6-D-Manp1�'、�'3-L-Araf1�'、�'5-L-Araf1�'、�'2,4-L-Rhap1�'、�'3-L-Rhap1�'、T-D-Glup1�'、�'4,6-D-Glup1�'、T-D-Galp1�'、�'3-D-Galp1�'and�'6-D-Galp1�'. The T-D-Galp1�'in the percentage ofcases a maximum of22.35%. Tea flower polysaccharide acid the sugarTFPS2-2contains six kinds of residues, respectively, as follows:�' 2,6-D-Gluf1�'、�'4-D-Glup1�'、�'3-L-Rhap1�'、T-D-Galp1�'、�'2-L-Arap1�'and�'4-D-GalpA1�'. The�'4-D-GalpA1�'in the percentage ofcases a maximum of49.80%. Tea seed polysaccharide neutral sugarNTSPS1contain the four kinds of residues, respectively:T-D-Glup1�'、�'6-D-Galp1�'、�'3-L-Araf1�'and�'5-L-Araf1�'. The�'6-D-Galp1�'in the percentage of cases a maximum of45.64%.Sodium periodate oxidation and Smith degradation reaction on TFPS1,experimental results are with TFPS1the methylation analysis results areconsistent, can be identified in this article methylation analysis methodsand experimental results are reliable. The TFPS1and TFPS2-2NMRspectra, combined with the preliminary experimental results, the initialguess to get TFPS1, and TFPS2-2may be a chemical structure.(2) The use of CD circular dichroism to study the source of teapolysaccharide NTPS ATPS2, TFPS1TFPS2-2solution in the high-levelstructure of the polysaccharide molecules with the changes of externalconditions arising from the change. The experimental results show thatchanges in pH, solution temperature changes, Calcium Ions,microwave heating and ultrasonic treatment of the tea solution (Flower)the conformation of the polysaccharide molecules and molecularstretching have a certain impact.(3) Tea (flower) polysaccharides were sulfated as well as calcium,iron, zinc, selenium coordination reaction of chemical modifiedsubstances IR analysis, and DSC analysis, and combined with thecomprehensive analysis of the ICP, that: Tea (Flower) thepolysaccharide been related to chemical treatment, chemicalstructure has been a slight change, that is tea (flower) hydroxyl groupson the polysaccharide structure has been involved in chemicalreactions vary, which can be identified tea (flower) polysaccharidebeen related to chemical change The experiment has been generated,corresponding to the new compounds. ICP, the weight percent of the coordination compounds of calcium, iron, zinc, and selenium in teapolysaccharide complexation elements as follows:2.7%,1.06%,0.097%,4.36%; tea flower polysaccharide calcium, iron, weight percent zinc,selenium coordination compounds complexing elements:3.16%,0.392%,18.7%,0.122%.(4)The α-glucosidase glucosidase activity inhibitory rateexperimental results show that: the activity of the source of teapolysaccharide extracted from different parts of the tea is differentthe.Tea polysaccharides on the alpha-glucosidase activity inhibitory rate isrelatively high, the tea flower polysaccharides on thealpha-glucosidase activityinhibitory rate is relatively low. Acidic sugar with the tea sourcepolysaccharide isolated α-glucosidase activity inhibition rate higherthan neutral sugar, this may be due to higher acidic sugar galacturonicacid content, the α-glucosidase enzyme activity inhibition is higher.