Alcoholic Liver Disease Resistance Of Traditional Chinese Medicine Syndrome And The Mechanism Of Action Of Alcoholic Liver Fibrosis

Objective: Alcoholic liver disease (ALD) is the toxic liver injury and a serious of diseases caused by drinking for long time, which is the clinical common disease. By means of the methods and doctrines of Clinical Epidemiological Research, we aim at the investigation of the characteristics of traditional Chinese medical syndrome distribution of alcoholic liver disease, and its correlation with clinical data. To explore the mechanism research of intervention target of traditional Chinese medicine compounds, we evaluated its effectiveness on alcoholic liver fibrosis by observing the rat models of alcoholic liver fibrosis.Methods:1. Theoretical study: Ⅰ summarized the cognition of the cause and mechanisms of alcoholic liver fibrosis by the angle of traditional Chinese medicine, and the clinical research on the Chinese medicine treatment of ALD. On the other hand, I expounded the pathogenesis of ALD by the angle of western medicine.2. Clinical investigation: We use statistical software to analyse clinical information; use factor analysis method to analyse and discuss the TCM Hou distribution of ALD, and the influencing factors.3. Experimental research: Divided the male Wistar into two groups randomly: the blank control group and model group. The rats of the model group were fed of the compound in the proportion of Distilled spirit、Vegetable oil、Qyrazole to make animal models of ALF, which cost used16weeks. When finished, the animal models were divided randomly into five groups:model group、model inverse group、positive drug treatment group、high dose Manganxiao treatment group and low dose Manganxiao treatment group. After three weeks in treatment group, we picked the serums respectively to detect the content of aspertate aminotransferase (AST), alanine transaminase (ALT), albumin, total protein, Ⅰ collagen type, laminin protein(LN), hyaluronic acid, Ⅰ type collagen amino end before before Ⅰ peptides, collagen type cross-linking end carboxyl peptide, Ⅲ type collagen protein and fiber combination before protein; liver tissues were embedded generally by olefins, sliced up and dyed by HE and Mallory. And finally, we took statistics that resulted from the serology test with the software SPSS18.0, and the pathological image to get the mean and make unit factor analysis of variance. Result and Conclusion:1. Clinical research: in the collection of cases, the male and female ratio is6:1at the age of35to44years and45to54years old. In the two age groups, Daily alcohol consumption is80-119g/d, drinking fixed number of year is10-19years. When GGT in50-89u/L, incidence of alcoholic liver disease is the highest. Syndrome type of alcoholic liver disease patients is mainly Stagnation and Spleen Deficiency, Dampness and Heat, phlegm and blood stasis, Phlegm and Dampness Resistance, and1iver and kidney deficiency. The proportion of Stagnation and Spleen Deficiency, Dampness and Heat is the most. Age, drinking fixed number of year, AST/ALT value to distribution characteristics of the alcoholic liver disease of traditional Chinese medical syndrome have influence, and have significant difference (p<0.05). And body mass index, daily alcohol consumption to syndromes of alcoholic liver disease had no effect on the distribution. Preliminary showed that the TCM syndrome of alcoholic liver disease is closely related with liver damage. Experimental study: content of glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease(AST), I collagen type, Laminin protein, Hyaluronic acid, I before the collagen amino type before peptides, I collagen type cross-linking end carboxyl peptide, Fibronectin (FN) in serum of modelset rats is significantly rised(p<0.05or p<0.01). Total protein, albumin, III before collagen type of serum have no significant change (p>0.05). In the liver tissue, a lot of collagen is deposited, it has a characteristic of liver f ibrosis pathological characteristics, content of glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease(AST), I collagen type, Laminin protein, Hyaluronic acid, I before the collagen amino type before peptides, I collagen type cross-linking end carboxyl peptide, Fibronectin (FN) in serum of Medication Group rats is significantly reduced (p<0.05or p<0.01). In the liver tissue, collagen is decreased greatly, the structure of the hepatic lobule is essential complete, has not seen the false flocculus. Immunohistochemistry results shows, the liver tissue of modelset rats after PCNA dyeing, we can see a large number proliferous nucleus, positive reaction particles of I collagen type and IV collagen type and Laminin protein(LN) is significantly increased.After medical treatment, the liver tissue after PCNA dyeing, we can see proliferous nucleus is reduced, positive reaction particles of I collagen type and IV collagen type and Laminin protein(LN) is significantly decreased. This shows that the Manganxiao can protect cytomembrane, it can inhibit excessive prol iferation of nucleus, i t can effective impact in I col lagen type, IV collagen type, Laminin protein, Hyaluronic acid, I before the collagen amino type before peptides, I collagen type cross-linking end carboxyl peptide Fibronectin (FN), so it can inhibit the deposit in the extracellular matrix, thereby it can inhibit Liver fibrosis.