Life Of Fetal Pill On Repeated Spontaneous Abortion Model Mice Uterine Decidua Hsp27 And Tf Expression Effect

Objective:Detection of repeated birth pill of natural abortion mice organization HSP27membrane and the influence of the Tf expression, and discusses the role of Shou Taiwan.Method:Will only50female CBA/J mice respectively with the20males DBA/2and5only BALB/c by2-1and cage in mice, mating, inspection to the Yin bolt for pregnancy plan0d, repeatedly natural abortion model respectively established40with a normal pregnancy only10only model, according to the order of pregnancy randomly divided into model group, Shou Taiwan of high, medium and low dose group. Since the1d began to fill pregnancy stomach, a normal pregnancy group and the model group to give the same amount of saline fill the stomach, and the other three were given life tire pill suspension filling liquid stomach, until at14d executed mice, take exuviate membrane organization, and dual phase electrophoretic and mass spectrometry technology proteomic techniques to find out the differences and natural abortion related proteins HSP27, Tf, with protein mark and immunohistochemical experimental detection life to HSP27tire pill and the influence of the Tf protein expression. Result:1.With a normal pregnancy than the model group, and natural abortion mice with HSP27express the membrane (P<0.01). Longevity tire pill low, medium and high dose group all can reduce repeated natural abortion mice with the expression of the uterus HSP27membrane, and Recurrent abortion model a statistically significant difference in group (P <0.01), and the life of high dose group and in the womb pills in low dose group, compared with significant difference (P<0.01) and how life of high dose of tire pill of Recurrent abortion curative effect is better.2.Compared with normal uteruses vibration group, repeated natural abortion model of mice uterine film Tf significantly down expression (P<0.01). Life of high dose group can tire pill Recurrent abortion mice increased the expression of the Tf uterine metamorphosis membrane (P<0.01); Life in the womb, low dose group shot of uterine exuviate membrane Tf expression and Recurrent abortion model group compared with no significant difference (P>0.05); Life of high dose group and in the womb pills in low dose group, compared with significant difference (P<0.01).Conclusion:Longevity tire pill can reduce miscarriage by a mouse model HSP27film the expression of the uterus and the expression of the Tf rise, make the pregnancy is maintained, and so to defend role.