Experimental Study On Shou Taiwan's Interference Of The Protein Expression Of Socs In Maternal-fetal Interface Of Mouse Models With Rsa

Objective:To explore the maternal-fetal regulation mechanism of immunization under the therapy of Shou Taiwan in recurrent spontaneous abortion (RSA) via detecting:①the expressive differences of protein in such maternal-fetal interfaces of mouse models with RSA as SOCS1, SOCS2 and SOCS3;②he effects of Shou Taiwan on the protein expression of SOCS1 and SOCS3 in maternal-fetal interface of mice with RSA。Method:CBA/J×BALB/C and CBA/J×DBA/2 were used as Normal pregnancy and RSA model respectively. The protein expressions of SOCS1, SOCS2 and SOCS3 in decidual and placental tissues on day 14 of gestation were detected by immunohistochemistry. The mice were assigned to Normal pregnancy group, model group and low, middle, high dose group of Shou Taiwan. After 14 days of administration, the protein expressions of SOCS1 and SOCS3 in mice decidual and placental tissues were detected by immunohistochemistry. Result:1. Compared with Normal pregnancy model mice,the expression of SOCS1 had significantly increased (P<0.05) and the expression of SOCS3 had significantly decreased in RSA model mice (P<0.01).The expression of SOCS2 had no obvious differences between normal pregnancy and RSA mice(P>0.05).2. All groups of Shou Taiwan could decreased the expression of SOCS1(P<0.01).There were no obvious differences between Normal pregnancy and all groups of Shou Taiwan(P>0.05). Middle and high groups of Shou Taiwan could increased the expression of SOCS3 (P<0.05andP<0.01). There were no obvious differences between Normal pregnancy and high dose group of Shou Taiwan(P>0.05).Conclusion:The molecular mechanism in the therapy of RSA by Shoutaiwan maybe is that,Shou Taiwan reduce the expression of SOCS1, raise the expression of SOCS3 on decidua of mouses with recurrent spontaneous abortion and correct the unbalance of Thl/Th2 on maternal-fetal interface.