| [Objective]: Learning and memory is the basic function of the brain, the people of thepast experience through the learning and memory put these experience to image stored inthe hippocampus way medial temporal lobe and they all formed between the neuralnetwork, the structure of ischemic tolerance is poor, so I through the establishment ofcomplete cerebral ischemia in rats model, some different cerebral ischemia in rats long tothe memory and then the learning ability of the influence, and understand cerebralischemia in patients after therapy, learning ability, for the follow-up of patients withcerebral ischemia rehabilitation treatment provides the basis[Method]: In the study, the improved pulsinelli four vessels ligation method for thepreparation complete cerebral ischemia in rats model, will only healthy adult32Wister ratsrandomly divided into four groups，Group1(JiaShouShu group), group2(cerebralischemia3min group), group3(cerebral ischemia5min group), group4(cerebralischemia10min group)，Make die in before and after, using Morris water maze of fourgroups were training in rats in two stages of learning and memory training, through to thegroup of rats study result analysis, evaluation ischemia changes before and after learningability and then by immunohistochemical detection rats histological change behavior[Results]:1.JiaShouShu group HE dyeing results show that CA1neural cells in the hippocampusarea aligned rules, cytoplasmic red dye uniformity, form, quantity and distribution of normal, cell layer can beclearly seen, its internal bigger and the nucleus of a round, nucleoli are clearly visible. Ischemia group HEdyeing visible the hippocampus cone cells are arranged the chaos, cell volume change, cell producesdenaturation, ischemia edema, cell density also change to appear empty bubble, organelles appears dissolvedphenomenon, and the imagination as ischemia time is extended increase trend.2. JiaShouShu group of big rathippocampal CA1area of so many visible NR1positive expression, cerebral ischemia3min,5min and10min group CA1area in the hippocampus NR1immune positive of the strength of PU value is dyeingJiaShouShu group NR1dyeing of PU value positive strength declined obviously (down22.12%~35.36%),explain with ischemia time NR1extended express positive declined and is more apparent, this mechanismmay be in the brain ischemia a protective mechanism. [Conclusion]:1.Through the water maze testing can see, ischemia model group compared with JiaShouShu group,made before learning ability groups mold has no obvious difference, made the JiaShouShu leader when the memory and then theability to learn more ischemia group high, cerebral ischemia in rats from extended the incubation period, through fewer platformlearning ability again dropped significantly, learning ability with the extension of ischemia time decreases, and ischemia rats againafter the training and long-term memory and learning ability is also lower before.2. Use the burning after improving measures manufacturing four artery occlusion whole brain ischemia model method canmake made mode are improved greatly success rate, is the study of ischemic disease is more secure and reliable model.3. NMDA receptors in each group model with ischemia time ischemia extend the decrease in thenumber of, through the water maze testing learning and memory ability to drop, that the NMDA receptors inthe learning and memory formation.4. Through the experiment, from the macro and micro discusses different duration of cerebral ischemiain rats with the memory and learning ability effect, and cerebral ischemia in patients of reaction in social lifeafter learning ability, their learning ability and positively related to the degree and illness. |
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