On The Heat Detoxification Scattered Stasis Party Non-infectious Rats Serum Endogenous Pyrogens

Object:To construct non infective fever SD rat model,To exploring the changes of the tumor necrosis factor alpha (TNF-a)、IL-1β、COX-2in the Plasma in the synovial of the collagen II induced arthritis (CIA) in rats, and explore the role of TNF-a、IL-1and COX-2in rheumatoid arthritis; To observe the influence of the Qiresanyujiedufang on TNF-a^IL-1β、COX-2Concentrations in the plasma, and explore the mechanism of the Qinresanyujiedufang in the treatment of rheumatoid arthritis disease.Methods:we Select60SPF level SD rats with6weeks of age and randomly selected6among the rats as the saline control group (blank control group, n=6); Elected50from the rest of the other rats and subcutaneous injection2,4-dinitrophenol induced endogenous fever model. Selected42successful model and then randomly divided into three groups and numbered them:①the heating model group (model group,n=12);②The Qingresanyujiedufang group (Chinese medicines group, n=12);③The western medicine naproxen treatment group (western medicine, n=12); three group rats are subcutaneous injection2,4-Dinitrophenol to induce endogenous fever model. Then give the group of western medicine group and TCM rats drug intervention immediately. Normal group and model group were0.9%saline treatment. Each group in order to measure the body temperature and determination the content of NF-a, IL-1β,and COX-2in serum by ELISA after2h. Recorded and compared2h rats’body temperature and index Content in serum in each group. evaluation treatment effect of endogenous fever treat by Qiresanyujiedufang based on the results of comparing.Result:(1) Temperature contrast in group after modeling2h:After modeling2h The body temperature of model, traditional Chinese medicine group and western medicine group were significantly higher than the The blank group, and the difference was significant (P<0.01).(2)Linear correlation between the three detection indicators and body temperature, and the difference was statistically significant.(P=0.01)(3) The observation indexes in serum contrast in group after modeling2h:After modeling2h The observation indexes in serum of model, traditional Chinese medicine group and western medicine group were significantly higher than the The blank group, and the difference was significant (P<0.01).Conclusion:(1)TNF-a, IL-1, and COX-2is an important mechanism what leading cause of endogenous beta (non infective fever).(2)Qingresanyujiedufang can effectively reduce the non infective fever of body temperature in rats.(3)Qingresanyujiedufang reduce non infective fever rats body temperature have a continuing role.(4) Qingresanyujiedufang can lower serum TNF-a, IL-1beta, and the content of COX-2, Explain that Qingresanyujiedufang treating blood stasis type of carcinoma fever, may be has relation with and can reduce the cancer fever in sera of patients with TNF-a, IL-1beta, and COX-2.