| The weakness of five basic organs induces the diabete." This highlights the important of five basic organs in diabetes incidence.according years of the experience in clinical practice, Pro. gao put forward a thought of syndrome differentiation from the liver-spleen-kidney on T2DM treatment, and he thought:Characteristics of the pathogenesis of type2diabetes is the liver, spleen and kidney, which are sick at the same time, for whatever reason-any one injuries in the liver, spleen, kidney, is gradually spread to other two organs, resulting in three organs disease, as well as stagnation of qi, wet muddy, blood stasis, and inner heat. Therefore, The treatment should coordinate the three organs’ relationship and prioritize and cure three organs simultaneously. Based on the theory and the new model of "syndrome differentiation from the liver-spleen-kidney on T2DM treatment", three prescriptions are prescribed by Pro. Gao. Glucose-lowering1:given priority to notifying kidney and calm the liver, accompanied by nourishing spleen, both qi and yin, and dispelling the heat and dampness, invigorating blood circulation; Glucose-lowering2:given priority to soothing the liver, accompanied by nourishing spleen and kidney, both qi and yin, and dispelling the heat and dampness, invigorating blood circulation; Glucose-lowering3:given priority to nourishing the spleen, accompanied by soothing the liver and nourishing kidney, both qi and yin, and dispelling the heat and dampness, invigorating blood circulation. In order to study the effectiveness of these three prescriptions about hypoglycemic mechanism, we performed the following experiments:1Fed with high fat diet combined with intraperitoneal injection of streptozotocin (STZ) to build a rat model of type2diabetes, by measuring the blood sugar, glycosylated hemoglobin, blood lipid, insulin and insulin sensitivity index (ISI) to observe the influence of the glucose-lowering1and2for treating experimental diabetic rats. The result: glucose-lowering1and2can reduce blood sugar and triglycerides, improve glucose tolerance and increase insulin sensitivity of diabetic rats significantly, showing good hypoglycemic effect, and glucose-lowering1is better than2.We found that after the drug intervention for4weeks, the Rats’blood glucose and lipid is reduced, but insulin levels did not increase, and the hypoglycemic effects may associate with the increasing of insulin sensitivity in rats, and the lipid-lowering effect may be associated with hypoglycemic effect, the glucose-lowering3has the tendency of hypoglycemic action and improving glucose tolerance, while compared with model group, there was no significant difference.2using medicine rat serum of glucose-lowering1to intervent C2C12skeletal muscle cells, which is differentiated after the mature with the palmitic acid and the calf serum albumin DMEM to be intervented to establish IR module, then divided into normal control group, the IR model group, sugar high, medium and low dose group and positive drug metformin group, eight replicate well in each group. After drug intervention for24h, take supernatant for determination of inflammatory factor, and scrape cells, to detect RNA, protein extraction or streaming respectively. The result showed that expression quantity of PI-3k p85, Akt, GLUT4, IRS-1mRNA of IR model, lower than normal cells, while higher after being intervented by drug-containing serum stimulus for24h. drug-containing serum can stimulate C2C12cells to increase PI-3k p85, Akt, GLUT4, IRS-1gene. It prompts drug-containing serum probably affect the level of GLUT4and IRS-1gene to improve insulin resistance by regulating the PI3K/AKT signal pathway. |
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