| Through the establishment of alcohol-induced rat model of acute gastric injury, the pharmacodynamics, Pathomorphology study Chinese medicine of different therapies and the effect of the compound on the prevention and treatment of acute alcoholic gastric lesions, and thus to clarify TCMGoverning Law and mechanism of action of the compound on acute gastric lesions. Provide an objective basis for the clinical treatment of gastric mucosal injury class of diseases.In this experiment,8-week-old male Wistar rats of144, weight (170±10) g. Were randomly divided into8groups, groups for the control group, model group, positive control group, groups of CHSGS, clear soup group, laughing San danshenyin on the group, security and pill group, Huangqijianzhong soup group. Each group was under15,30,60minutes,3hours group, every time the group of six. Each group were administered for7days, once a day.7days in addition to the normal group, the other groups, respectively, with56%ethanol10ml/kg gavage modeling, modeling at15,30,60minutes to3period, anesthetized rats drawn.The assay method for biochemical determination of total acidity and mucus protein content, visual observation of gastric ulcer damage, count the ulcer index. Paraffin sections, HE staining was observed pathological morphological damage and mucosal inflammatory cells and mucosal cell count. ELASE determination of gastric mucosal tissue homogenates IL2, IL6, IL10, TNF, EGF content changes.These results suggest that ethanol induced gastric lesion index, gastric secretion, total acidity of gastric juice, gastric mucus protein, the number of inflammatory cells in15minutes,30minutes Paul and pills in general intervention effect, after60minutes of Chinese medicinegroups have a role, especial ly Sal via drink and Shixiao scattered group, soup Huangqi jianzhong group role significantly. Digestion dredge acute gastric mucosal injury early application can play a positive role in the protection, the initial prescription of gastric mucosal injury-like illness. In the late can be applied to blood circulation, temperature in the spleen, which is also in line with the law of the incidence of stomach pain, namely:early fresh wounds, in the late injury blood wound spleen. This experiment the influence of alcohol IL2, IL6, IL10, TNF, EGF, Bupleurum CHSGS Salvia drink together Shixiaosan, Huangqijianzhong soup can increase to15minutes,30minutes,60minutes, the gastric juice IL2content. Paul and pill group30minutes gastric juice IL6highest content. Astragalus Decoction and Salvia drink together vector laugh scattered significantly reduce the influence of alcohol15minutes gastric juice IL10content. Alcohol injury in30,60minutes, the Recipe rats gastric juice I LI10levels were significantly reduced, in which the role and stronger Salvia drink together vector laugh bulk. Various herbs in alcohol for15minutes gastric juice levels of TNF were significantly lower compared with the rat model, P<0.05, strongest role which Huangqi jianzhong soup. Various herbs in alcohol for30minutes gastric juice levels of TNF were significantly reduced, and the rat model comparison P<0.05, which Salvia drink together vector laugh strongest scattered role. Various herbs in alcohol for60minutes gastric juice TNF levels were significantly lower compared with the rat model, P<0.05, strongest role which Huangqijianzhong soup. The security and pills and CHSGS a significant increase in the influence of alcohol30minutes after the gastric juice EGF levels with the model rats (P<0.05). Clear soup and CHSGS a significant increase in the influence of alcohol after60minutes EGF content of gastric juice with the model rats (P<0.05).In summary and scholars’research, reported in the literature is mostly in the single herbs, Chinese herbal formula, herbal extracts, research limited to a single disease, the experiment from digestion dredge, blood circulation, liver qi, Qingrehumidity, temperature in the spleen and other aspects of the law and protection mechanisms of Chinese medicine to protect the gastric mucosal injury card, provide a scientific basis for the clinical treatment of the disease. The results suggest that:traditional Chinese medicine group compared with model group have certain stomach injury situation caused by alcohol prevention and treatment, from which we have come to the law, ethanol induced gastric mucosal injury due to the scope of Chinese fresh wounds, combined with ethanol characteristics, and thus hurt the blood, spleen and stomach injuries. |
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