Study Of New Chiral Separation Medium

The preparation of BSA-CSP capillary monolithic column involved in situ synthesize of the GMA-EDMA stationary phase matrix in the capillary followed by its surface modification using BSA with a certain conformation. The evaluation of the BSA-CSP capillary monolithic column by CEC was accomplished by separating the enantiomers of tryptophan/phenylalanine/histidine or glycine under the the optimization of experimental conditions. Especially, the resolution of the phenylalanine enantiomers was over8.8. In addition, the BSA-CSP capillary monolithic column was successfully applied in the separation of five chiral drugs, i.e. benzoin, cevimeline, warfarin, oxybutynin and mexiletine. Especially, the resolution of cevimeline was observed more than7.7. Furthermore, the BSA-CSP for capillary monolithic column was also used to separate gastrodiae extract by5mmol/L-10%ACN-pH=7.1phosphate buffer according to the principle of biology chromatography. Different retention time was observed for different components, indicating the ability for the separation of bioactive materials using the BSA-CSP for capillary monolithic column.Vancomycin silica microsphere stationary phase was successfully prepared after the surface modification of silica and the exploration of the bonding effect under different operating conditions. Using Vancomycin silica microsphere stationary phase on capillary column and liquid chromatography column, four amino acids, i.e. se-methyl-selenocysteine, phenylalanine, tryptophan and glutamic acid, were separated. Resolutions were larger than4.2for all samples, indicating the high resolution for chiral isolation using Vancomycin silica microsphere stationary phase.