Studies On The Effects Of Carbaryl And Its Major Degradation Product1-Naphthol On The Growth Effects Of Chlorella Vulgaris

As a representative products of Carbamate pesicides, Carbaryl can tag out and have stomach poisoning to insects. It also has suction effect, can inhibit the transmit of the pest’s nerve system cholinesterase leading to the pests die. The major degradation product of Carbaryl is1-Naphthol.1-Naphthol has strong irritating and poison effect on human skin, eye, blood and liver, and has potential carcinogenic, teratogenic, allergenic and mutagenic poison.1-Naphthol is a environmental pollutants. Aglae are very sensitive to environmental pollutants. Measureing and evaluating the effects of external contamination on the growth of algae, which can reflect that the contamination has the possible comprehensive effects on primary products in the aquatic ecosystems. So we choosed Chlorella vulgar is as experimental objects in this artical, studied on the effects of Carbaryl and its major degradation product1-Naphthol on the contents of biomass, Chlorophyll, malonaldehyde(MDA) and soluble protein of C. Vulgaris. The specific results are as follows:To C. Vulgaris, Carbaryl and1-Naphthol are both high-toxic. After7days C. Vulgaris had been nurtured in BG-11with Carbaryl and1-Naphthol respectively, we found that the higher the both drugs’concentration were, the greater toxicity they would be to C. Vulgaris. It appeared obviously dose-effect relationship. The96h EC50of Carbaryl to C. Vulgaris was2.50mg/L, meanwhile the96h EC50of1-Naphthol to C. Vulgaris was3.10mg/L. At the same level, Carbaryl has greater toxicity to C. Vulgaris than1-Naphthol.According to the effects of Carbaryl and1-Naphthol on biomass contant of C. Vulgaris, we studied the effects of Carbaryl and1-Naphthol on the chlorophyll contant of C. Vulgaris just seperated the concentration of these two drugs into three different gradients (1.0mg/L,5.0mg/L,10.0mg/L). The low and moderate concentration of Carbaryl had greater influence on the chlorophyll of C. Vulgaris than1-Naphthol. However, the higher concentration of Carbaryl had greater inlfuence on the chlorophyll of C. Vulgaris after7days nurtured in the medium. This obviousely demonstrates that Carbaryl is unstable in the water environment, and the1-Naphthol has more sustainable toxicity to C. Vulgaris.Malondialdehyde (MDA) is a product of lipid peroxidation, it is also an important detection index for cell oxidative damage. This artical studied on the MDA content of C. Vulgaris after7days nurtured in the medium with Carbaryl and1-Naphthol respectively. We found that the MDA content of C. Vulgaris had been increasing with the gradually rising concentration of Carbaryl and1-Naphthol. The MDA content of C. Vulgaris with high concentration of Carbaryl treatments were much higher than the control group (P<0.01). On the other hand, even the1-Naphthol had promoted the increasing the MDA content of C. Vulgaris, the results had not appeared the significant differents with control group. It indicates1-Naphthol may have different physiological and biochemical mechanisms effects on C. Vulgaris compared with Carbaryl.Exposured to the different concentration of Carbaryl and1-Naphthol, respectively. The soluble protein content of C. Vulgaris appeared increased firstly and then decreased. The1.0mg/L and5.0mg/L of Carbaryl and1-Naphthol could promote the protein content of C. Vulgaris, but the10.0mg/L concentration of Carbaryl and1-Naphthol inhibit. At the same time, the soluble protein content of C. Vulgaris with10.0mg/L of Carbaryl and1-Naphthol was of significant different with control groups(P<0.05). But, by contrast, the10.0mg/L of Carbaryl treatments were not. It indicates that high concentration of1-Naphthol has much greater effects on the soluble protein content of C. Vulgaris.To sum up, begin with aspects of the biomass content and physiology and biochemistry of C. Vulgaris, we studied on the effects of Carbaryl and1-Naphthol on the C. Vulgaris. What we have found is that Carbaryl and1-Naphthol can influence the biomass content, chlorophyll, MDA, soluble protein content of C. Vulgaris. They both have certain toxicity to C. Vulgaris. All we have studied provide a basis scientific theory data for reasonable evaluating, managing, controlling the pollution effects of these both substances.