Research On The Toxicity Effects Of BPA On Chlorella And Zebrafish

BPA （Bisphenol A） is widely used in feeder-bottle, food packing and medicalinstruments. It had negative ecological effects on human health and theenvironment.This study chosed Chlorella and Zebrafish as the research objects tostudy the ecological effects of BPA on them, and provided scientific basis tounderstand ecological toxicity effect of BPA on aquatic organisms.According to the mastery of the growth cycle of Chlorella, the acutetoxicitu,celluar porphology,growth state and some biomacromolecule were done tostudy the effects of BPA on Chlorella.The results were as follows.The96h EC₅₀ofBPA was8.453mg/L. The Chlorella had the certain ability of enrichment ofBPA.The higher intial concentrarions of BPA and the longer of time,the greateramount of enrichment.On the45thday,the maximum amount of enrichment and BCFof BPA were respectively0.205mg/L and9.70%.Howerer,Chlorella had the ability ofdegradation.BPA of4mg/L had promoting effects on the early growth ofChlorella.BPA of8mg/L had inhibitory effects on Chlorella.BPA made Chlorellacells morphogenesis spin or deformity.BPA also destroyed the Chlorella cellsmembrane and its permeability. The higher concentrations of BPA,the stronger thiskind of demage.The content of polysaccharides and chlorophyll were effected byBPA.The higher concentrations of BPA,the lower content of polysaccharide andChlorophyll.BPA of10mg/L induced first and inhibited the viability of Chlorella.Chlorella was oxidative damaged by BPA of12mg/L,and the viability of Chlorellawas inhibited and decreased.Through the actue toxicity study on Zebrafish, ecological safety of BPA wasanlysed.This article studied the injury of organ, genetic substance （DNA） andcatalase.The main conclusions were as follows.96h LC₅₀of BPA on Zebrafish was9.04mg/L, the Zebrafish was a median toxic substance to Zebrafish,and the safeconcentration of BPA was0.904mg/L. BPA made adult Zebrafish poisoning, but hadnot obvious effects on the quality of mature Zebrafish.BPA of1/4LC₅₀had toxicityeffects on the liver of Zebrafish.With the increase of BPA concentrations,the stronger of toxicity effects.BPA of1/6LC₅₀could induced micronucleus in peripheral blooderythrocyte.BPA had mutation function on red blood cell.With the concentrations ofBPA increase,the mutagenic effect increased. BPA induced the Zebrafish liver andgills CAT activity peak in advance. Zebrafish liver (1/3LC₅₀) of low concentrationsof BPA was relatively sensitive, gills were more sensitive to high concentrations (1/2LC₅₀) of BPA.Results of this study provided a scientific and reliable theoretical support in riskassessment and ecological toxicity effects of aquatic organisms.