Construction Of SERS-active Substrates Based On The Microfiltration Membrane And Their Properties And Applications

Surface-enhanced Raman scattering (SERS) has attracted considerable attention becauseit not only keeps the fingerprint character of Raman spectroscopy, but also enable to achievesingle-molecule detection. Nowadays, the studies of SERS are not limited to the noble metalnanoparticles and a piece of glass or Si wafer, but other in-depth aspects such as themechanism and properties of enhancement. As the most important part of SERS, more andmore efforts are underway to prepare the SERS-active substrates. How to prepare an excellentsubstrate to extend this analytical tool to other scopes such as bioscience, medical science,environmental monitoring, and food security, has been one of the most meaningful field ofSERS study. The performance of SERS substrates mainly depends on the materials,morphology and the adsorption performance of substrates. Based on the research background,this thesis has focused on the preparation of the materials, the optimization of SERSsubstrates, and their practical applications in detection. The substrates fabricated in this studydemonstrated both excellent performance and practical effect. The major contributions of thiswork are as follows:(1) An environmentally friendly synthesis method was developed based on theseed-mediated growth and silver mirror reaction to get the narrow dispersed silvernanoparticles with controllable sizes range from20nm to120nm.(2) A preparation method was been proposed based on filter membrane and optimizedaccording to the actual demands. Moreover, the calculation method of enhanced factor (EF)was optimized by the quantification of the probe molecules.(3) Combination of the filter-based substrates and solid-phase extraction (SPE) for tracedetection. The combination of adsorptive preconcentration and specific adsorption made theSPE-SERS mode available and useful in practical detection area.