Preparations Of Flavonoids Of Ginkgo Biloba Leaf For Injection

Flavonoids of Ginkgo leaves are very important to extracts of ginkgobiloba(GBE).The mechanism of main pharmacology is believed to beproduced by its functions as a neuroprotective agent, an antioxidant,afree-radical scavenger, a membrane stabilizer. Other pharmacologiceffects include the following: endothelium relaxation mediated byinhibition of 3,5-cyclic GMP(guanosine monophosphate)phosphodiesterase; inhibition of age-related loss of muscarinergiccholinoceptors andα-adrenoceptors;and stimulation of choline uptake inthe hippocampus, also has been shown to inhibitβ-amyloid deposition.In clinical research, it is used to treat the symptoms of early-stageAlzheimer’s disease, vascular dementia, peripheral claudication,andtinnitus of vascular origin.However, the way of both extraction process and purification aresignificantly variable to different manufacturers which result inunstability of pharmacologic efficacy.That leads to be difficulty toevaluate sysmatically efficacy.So it is necessary to establish the standardsfor procedures of extraction, refinement and the methods of qualitycontrol.The aim of this article mainly solve the extraction and refinementof flavonoid glycosides from ginkgo leaves.we adopt ethanol extractionaccording to polarity of flavone glycosides.The two kinds of absorptionresins which have been connected in series have been applied to refinerude extraction from ginkgo biloba leaves.We optimize the parameters ofapplication to absorption resin and render creatively application ofconnection in series for two kinds of absorption resin.The other aim is to develop a set of methods for quality control ofboth ginkgo biloba leaf and flovaloids of ginkgo biloba. The system ofacid water-acetonitrile linear gradient elution has been adopted to isolateflavonoids.Therefore, two kinds of chromatography fingerprints havebeen established.