Influence Of Compatibility Of Astragalus Polysaccharides And Rhein On Renal Tissue Apoptosis In Rats With Chronic Renal Failure

ObjectiveTo explore the mechanism of Astragalus Polysaccharide (APS) combined with Rheinon chronic renal failure(CRF) by studying the two drugs’ effect on endoplasmicreticulum stress(ERS)-induced apoptosis.Methods37male Wistar rats were randomly divided into normal group(N group), modelgroup(M group), low-dose group of APS (200mg/kg﹒d) combined with Rhein(25mg/kg﹒d)(L group), high-dose group of APS (400mg/kg﹒d) combined withRhein (100mg/kg﹒d)(H group). Wstar rats were fed with adenine (200mg/kg﹒d)by gastric tube to establish CRF model. After7weeks of treatment, renal function,serum electrolyte concentrations and pathological changes of all groups wereexamined, and Western-blot method was used to detect the expression of C/EBPhomologous protein（CHOP）, inositol-requiring protein-1（IRE1）,c-Jun-NH2-terminal kinase（JNK）in renal tissues.Results1. Kidney weight and kidney index: compared with N group, Kidney weight andkidney index of M group increased (P<0.01); compared with M group, Kidney weightand kidney index of L and H group decreased significantly (P<0.01).2. Biochemical parameters: compared with N group, BUN and SCr value of M groupincreased (P<0.01); compared with M group, BUN and SCr values of L and H groupwere significantly reduced (P<0.01); compared with N group, concentration ofcalcium ion of M group decreased (P<0.05); compared with M group, concentrationof calcium ion of H group was higher (P<0.05); compared with N group, phosphorusconcentration in serum of M group increased (P<0.01); compared with M group,phosphorus concentration in serum of L and H group decreased significantly(P<0.01). 3. Effect on renal tubular apoptosis: compared with N group, amount of apoptosiscells in renal tubular of M group increased (P<0.01); compared with M group, amountof apoptosis cells in renal tubular of L and H group were reduce (P<0.01).4. Protein expression of CHOP，IRE1and JNK: compared with N group, proteinexpression of CHOP，IRE1and JNK of M group increased (P<0.05); compared withM group, protein expression of CHOP，IRE1and JNK of L and H group decreased(P<0.05).ConclusionAPS combined with Rhein may alleviate renal cell apoptosis induced by reaction ofERS through reducing CHOP, IRE1, JNK expression in the rats with CRF, and thusplay a protective role for kidney.