BMSCs Biofunctional Effects Of Ti-6Al-7Nb Alloy Treated By HF+anodic Oxidation

Objective:Fast for the sake of early implant osseointegration and improve the primary stabilityof the implant surface treatment technology rapid development. Currently, nanoscalesurface treatment method has proved conducive to the implant surface cell adhesion andproliferation, is the research focus of the surface treatment method. Ti-6Al-7Nb alloy is anovel titanium alloy; Nb element is replaced by V element in the conventional Ti-6Al-4V,reduced toxicity, while the elastic modulus is closer to the bone tissue. In this study,surface morphology, chemical composition and characteristics of BMSCs the HF etching+anodized commercially pure titanium alloy Ti-6Al-7Nb observed.Methods:1、Surface treatment: titanium and Ti-6Al-7Nb30samples, silicon carbide sandpaperpolished surface, were randomly divided into treatment group and control group (n=15),the two control groups not treated; two treatment group HF etching+anodized. Then byscanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray energydispersive spectroscopy (XPS) to observe the sample surface morphology, and analysisof the chemical composition.2、BMSCs cultured and identified: take2-week-old female SD rats were sacrificed andthe depth of anesthesia, isolated from the femur and tibia, go to the net soft tissue resection at both ends of metaphyseal bone marrow were washed with PBS, centrifuged,after the shock transferred to culture flasks by adding DMEM medium containing10%fetal bovine serum, placed50ml/LCO2,37°C incubator culture. After3d，wash cellswhich adherent and the medium was changed to continue to foster3d for liquid1. ByMTT method, osteogenic and adipogenic BMSCs were identified.3、Different surface BMSCs characteristics: Cultured4th generation BMSCs wereseeded in combination gold surface, SEM observation of cell morphology; MTT methodto detect the adhesion and proliferation of cells in the surface of the material; ALP kitdetection of ALPResults:1、Surface treatment: pure titanium and Ti-6Al-7Nb by HF etching+anodized SEMobservation of the visible surface of the uniform distribution of nano-tubular structure,AFM observation shows the uneven surface; XPS detected surface fluorine (F)compounds, aluminum (Al) elements mainly exist in stable form of Al2O3.2、BMSCs culture and identification: a2-week-old SD rats were sacrificed, extractedfrom the bone marrow of BMSCs strong growth, develop3-5d will be able to completelycover the bottom of culture flask to subculture. By MTT assay, osteogenic, adipogenicand alizarin red staining and oil red O staining, combined with the observed underinverted microscope and found that the BMSCs laboratory cultures of normal growthcurve has good osteogenic and adipogenic differentiation capacity, identification goodresults.3、Different surface BMSCs characteristics: BMSCs in the smooth pure titanium andTi-6Al-7Nb alloy surface morphology, adhesion, proliferation showed no significantdifferences. The HF etching+anodized titanium and Ti-6Al-7Nb alloy surface, BMSCsform no significant difference in cell morphology of the treated surface to besignificantly better than the control group; But I can see. Adhesion and proliferation ofBMSCs surface in the treatment group were significantly higher than that in the controlgroup, but between treatment groups titanium and Ti-6Al-7Nb, adhesion andproliferation of no significant differences. BMSCs adhesion, proliferation and ALP activity of the surface of the two treatment groups were significantly higher than thecontrol group (p <0.05); between the two materials, Ti-6Al-7Nb treated ALP activity wassignificantly higher than the titanium treatment group (p <0.05).Conclusion:The HF etching Ti-6Al-7Nb+anodized, can significantly promote the adhesion ofBMSCs proliferation and ALP activity. These results suggest that the surface of the alloyTi-6Al-7Nb HF etching+anodized combined and enhanced to improve early implantbone surface biological activity has a good effect.