The Influence Of Chronic Intermittent Hypoxia During Pregnancy On The Expression Of Myocardial Cell ENOS、 Hsp90and The Production Of Serum NO In Offspring Male Rats

Objective: Through the establishment of pregnant rats and4weeks of age male offspring of chronic intermittent hypoxia model，to study the influence of chronic intermittent hypoxia whichimitates sleep apnea syndrome on the expression of myocardialcell eNOS、Hsp90and the production of serum NO in Offspringmale rats, and investigate the potential mechanism of chronicintermittent hypoxia on the development of cardiovasculardiseases.Methods：16pregnant SD rats at7days pregnant were randomly dividedinto two groups：chronic intermittent hypoxia group（I group）and normoxic control group(N group)。 After delivery, when theoffsprings were fed to4weeks of age, two male offsprings were randomlyselected from the the offsprings of every pregnant rat. N group and Igroup offspring at this time were then randomly divided into normoxiccontrol group(C group) and chronic intermittent hypoxia group（H group）.Thus4groups were maternal: pregnant chronicintermittent hypoxia plus male offspring chronic intermittenthypoxia group (IH,n=8), pregnant chronic intermittent hypoxiaplus male offspring normoxia group(IC,n=8); pregnant normoxia plusmale offspring chronic intermittent hypoxia group (NH,n=8);pregnant normoxia plus male offspring normoxia group(NC,n=8). Maleoffspring rats experienced chronic intermittent hypoxia12weeks until16weeks of age. HE was used to observe pathologicalchanges of16weeks of age offspring heart,Immunohistochemistrywas used to detecte the protein expression of eNOS and Hsp90in myocardial cell,Nitrale reduetase was used to measure the concentration of serum NO.Results:1. Prenatal or offspring chronic intermittent hypoxiacan cause offspring rats myocardial cell volume increasedslightly,but no significant difference（P>0.05）;2、Prenatalchronic intermittent hypoxia or offspring chronic intermittenthypoxia can decrease the expression of offspring rats myocardialcell eNOS，the influence caused by prenatal chronic intermittenthypoxia could be aggravated significantly when offspring chronicintermittent hypoxia is imposed(P <0.05)；3、Prenatal chronicintermittent hypoxia can decrease the expression of offspringrats myocardial cell Hsp90， offspring chronic intermittenthypoxia can increase the expression of offspring rats myocardialcell Hsp90(P <0.05)；4、Prenatal chronic intermittent hypoxia oroffspring chronic intermittent hypoxia can decrease theproduction of offspring rats serum NO(P <0.05)，the influencecaused by prenatal chronic intermittent hypoxia could not beaggravated significantly when offspring chronic intermittenthypoxia is imposed(P＞0.05).Conclusions：Prenatal chronic intermittent hypoxia can decreasethe expression of offspring rats myocardial cell eNOS、Hsp90and the production of serum NO，offspring chronic intermittenthypoxia can decrease the expression of offspring rats myocardialcell eNOS and the production of serum NO，the influence of theexpression of offspring rats myocardial cell eNOS caused byprenatal chronic intermittent hypoxia could be aggravatedsignificantly when offspring chronic intermittent hypoxia is imposed,which could be the potential mechanisms of chronic intermittenthypoxia on the development of cardiovascular diseases whenoffspring adult.Offspring chronic intermittent hypoxia can increase the expression of offspring rats myocardial cell Hsp90which can strengthen the body resist to adverse environment.