Study On Proteomics And Bioinformatics Of The Echinococcus Granulosus Protoscoleces From Infected Humans

Objective: Isolate and identify proteins from Echinococcus granulosusprotoscoleces by using improved proteomics method on the basis of the earlyresearch and establish the datebase of proteomics database of Echinococcusgranulosus protoscoleces. The property and function of all identified protoscolecesproteins were analysed by bioinformatics, aimed to provide molecular basis forfinding new candidates for immunodiagnosis, new vaccine and target proteins ofclinical importance.Methods: In this study we present a proteomic and immunoproteomicanalysis of Echinococcus granulosus protoscoleces. The proteins in Echinococcusgranulosus protoscoleces were separated from infected individuals bytwo-dimensional electrophoresis separating technique that depends on bothisoelectric points and molecular weights, followed by MALDI-TOF MS analysis.All identified protoscoleces proteins were analysed by bioinformatics, includingKOG, GO and KEGG pathway.Results: By2-DE and MALDI-TOF MS ananlysis330protein spots wereseparated, and49proteinswere identified in protoscoleces. By bioinformaticanalysis we find that those proteins involved in cytoskeletal, metabolism, signaltransduction and material transport. Several proteins include14-3-3protein,antigen P29, Paramyosin and Fatty acid-binding protein have been successfullyshown to possess a good antigenicity and immunogenicity. There are also28 proteins was identified on immunoblot of protoscoleces. Proteins homologous to E.granulosus hydatid disease diagnostic antigen P-29,14-3-3protein and Myophilinwere detected as well in the immunoblots.Conclusion: The way of proteomic research in Echinococcus granulosus hasbeen found. Some of Echinococcus granulosus protoscoleces proteins have beenidentified which are involved in the movement, growth, development andregulation of the parasite.