| Purpose:Study of the change of serum T cell subsets in unexplained recurrent spontaneous abortion (URSA), and the change of Thl/Th2type cytokines levels. Also study the relationship between the changes and the different pregnancy outcomes.Methods:â‘ 33cases of URSA patients,154cases of normal pregnancy women and116cases of normal non-pregnant women:using Flow Cytometry (FCM) detecting the serum proportion of CD4+, CD8+, CD16+CD56+T cells subgroup and the ratio of CD4+/CD8+in the normal pregnancy group, normal non-pregnant group and URSA patients per month after miscarriage; Enzyme-Linked Immunosorbent (ELISA) method is used to detect serum level of IL-2, IL-10, IFN-y, IL-4and IL-6;â‘¡23cases of URSA patients with normal pregnancy:using FCM detecting the serum proportion of CD4+, CD8+, CD16+CD56+T cells and CD4+/CD8+before and after pregnancy; using ELISA detecting the serum levels of IL-2, IL-10, IFN-y, IL-4and IL-6before and after pregnancy;â‘¢URSA patients with different pregnancy outcomes (44cases of URSA patients in normal pregnancy,16cases of abortion again):using FCM detecting the serum proportion of CD4+, CD8+, CD16+CD56+T cells and CD4+/CD8+; using ELISA detecting the serum levels of IL-2, IL-10, IFN-y, IL-4and IL-6.Results:â‘ URSA group, normal pregnancy group and normal non-pregnant group: CD4+T lymphocytes and CD8+T lymphocytes, CD16+56+T lymphocytes were the highest in the1month after abortion group, and gradually decreases along with the change of time tend to be stable, the changes in first3months after miscarriage group were obviously different with normal pregnancy group and normal non-pregnant group (P<0.05), the ratio of CD4+/CD8+gradually decreases with time, but did not see obvious difference between groups (P>0.05). Serum levels of Thl cytokine IL-2and IFN-y were the highest in1month after abortion group, in the first3months after abortion they were decreased significantly, and gradually decreased along with the change of time tend to be stable, and Th2type cytokines IL-10, IL-4and IL-6were highest in normal pregnancy group, but in the URSA group, the highest level was in1month after abortion group, the first3month after abortion were down significantly, and gradually decreased along with the change of time tend to be stable.â‘¡URSA patients with normal pregnancy compared before and after pregnancy, the serum proportion of CD4+T lymphocytes, CD8+T lymphocyte and C16+CD56+T lymphocyte was significantly lower when the patients with normal pregnancy (P<0.05); the serum levels of Th1type cytokines IL-2and IFN-y in pregnancy stage were significantly lower (P<0.05), while Th2type cytokines IL-4was significantly increased (P<0.05);â‘¢URSA patients with pregnancy in different outcomes, the serum proportion of CD4+T lymphocytes, CD8+T lymphocyte and the C16+CD56+T lymphocyte was lower in normal pregnancy outcome than that in miscarriage outcome, but P>0.05; in abortion outcome the serum levels of Thl type cytokines IL-2and IFN-y were higher than these in normal pregnancy outcome (P<0.05), the Th2type cytokines IL-10and IL-4were lower (P<0.05).Conclusion:1. The increases of CD4+T lymphocytes, CD8+T lymphocyte, C16+CD56+T lymphocyte and CD4+/CD8+were associated with URSA, prompted that the change in T lymphocyte subsets is an important mechanism of miscarriage. Thl/Th2cytokine changes were related to the occurrence of URSA, tend to Th2type cytokines are conducive to the maintenance of normal pregnancy, tend to Thl cytokines may lead to miscarriage.2. URSA patients after miscarriage, the changes of T lymphocyte subgroups and Th1/Th2cytokine were significantly different in the first3months after miscarriage, finally leveled off, consider that the first3months after abortion is still a high-risk period, and are not conducive to pregnancy again.3. Monitor the serum proportion of T lymphocyte subsets and the serum level of Th1/Th2cytokines in URSA patients may predict the outcome of pregnancy. |
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