Study On The Impact To Bone Dissolve Surrounding Prosthesis Rats OPG/RANKL/RANK Signal Path In Yougui Recipe To Intervene

Objective:Through treat a rats model of bone disslove surrounding prosthesis, In the Yougui recipe intervention, To observe the Yougui recipe effect the important signal pathway OPG/RANKL/RANK of rat femur osteoclast, Explore the classical prescription Yougui recipe for warming and recuperating the Kidney-Yang in the prevention and treatment of prosthesis surrounding bone dissolve disease of molecular mechanism,and,Chinese medicine about " kidney dominating bone " theory.Methods:The sixty SD rats,aged eight weeks, were randomly divided into six groups:blank groups, model groups, normal dose Yougui recipe groups,double dose Yougui recipe groups,high dose Yougui recipe groups, salmon calcitonin acetate groups,and ten rats in each group. No treatments to the blank groups,and others through were injected with titanium-screw and polyethylene particles in their femurs to build bone disslove surrounding prosthesis models. The kinds dose of Yougui recipe groups drink the Yougui recipe through intragastric administration, Blank control group and model group also through intragastric administration give physiological saline, salmon calcitonin acetate groups through of gluteus maximus muscle injection give dosage. To give medicine ten weeks after the chloral hydrate anesthesia, abdominal aorta take blood. The serum biochemical instrument detection Ca(calcium)、P(phosphorus)、ALP (alkaline phosphatase) Serum level. With rat TRAP-5b ELISA kit detection tartrate-resistant acid phosphatase5b(TRAP-5b), With rat OPG(os-teopmtegerin) ELISA kit detection OPG、With rat RANKL (ligandof receptor activator of NF-kB) ELISA kit detection RANKL, With rat RANK((receptor activator of NF-kB)ELISA kit detection RANK. Take the rat bilateral femoral specimens were histopathology and imaging observation, and through the fluorescence quantitative PCR observation femoral IL-1, IL-6gene expression changes.Results:OPG in serum level, Except the low dose of Yougui recipe group, the other treatment groups are significantly higher than model control group (P<0.05). High dose of the Yougui recipe group OPG serum level is higher than the salmon calcitonin group; In the serum RANKL level, the treatment groups are significantly below the model control group (P <0.05); The serum RANKL level of the high dose Yougui recipe group is minimum; In the serum level of RANK, the treatment groups are significantly below the model control group (P<0.05); The serum RANKL level of the high dose Yougui recipe group is minimum; In Trap5b serum level, each treatment group significantly lower than model control group, and salmon calcitonin group of RANK serum level is minimum; In Ca, P, ALP serum level, the treatment group are higher than the model group, and a statistical difference (P<0.05); High dose Yougui recipe group are higher than the salmon calcitonin group, and a statistical difference (P<0.05); And salmon calcitonin group are higher than the low, medium dose Yougui recipe group, Among them in the ALP, P serum level, calcitonin group were significantly higher than low dose Yougui recipe group (P<0.05). Molybdenum target and HE staining results show that compared with model control group, Each treatment group rats of bone dissolution surrounding prosthesis all have different degrees of improvement performance. Among them with the high dose Yougui recipe group have the most obvious improvement. Quantitative PCR results show that the relative blank control group, model control group IL-1, IL-6gene expression appeared rise; In drug intervention, the treatment group IL-1, IL-6gene again appeared different degree of cut, among them with high dose Yougui recipe group cut the most obvious.Conclusion:The experiment confirmed the Yougui recipe can inhibit osteoclast formation, differentiation and bone resorption activity, promote osteogenesis, restrain inflammation around prosthesis, improve bone resorption surrounding prosthesis. The Yougui recipe may through ativate OPG/RANKL/RANK signal pathway of osteoblast-osteoclast coupling relationship, stimulate bone marrow regeneration, and then play a promoting bone growth and inhibiting bone resorption role.At the same time for the Chinese medicine "kidney dominating bone" theory provides a strong experimental support, also for clinical treatment of bone dissolve surrounding prosthesis and bone metabolic disease added new main force.