| Objective:To explore the relationship between ICAM-1gene rs281432and rs281437SNPs and severe premature coronary heart disease,we did this study,the results are as follows.Methods:Totally consecutive181cases of hospitalized patients were collected at the Seco-nd Hospital of Tian jin Medical University during May2011to December2012. All cases accepted coronary angiography, among which103cases were male,32-67years old and78cases were women,45-75years old. Patients were divided into two groups according to the result of coronary angiography.106cases were divided into the disease group(early three lesion group)75cases were male,31cases were female.75cases were divided into the control group (group with normal coronary artery),28cases were male,47cases were female.Inclusion criteria:Disease group:the onset age of CHD for male is less than55years old and female less than65years old; Severe three-vessel coronary artery lesions indicates the area stenosis of LAD, LCX and RCA or LM and RCA are all more than75percentages according to Quantitative coronary angiography. Normal group:the three coronary artery are all smooth without stenosis.For all selected objects, gender, age, family history and history of diabetes, stro-ke, hypertension, smoking were detaild taken and blood pressure, body, weight, heig-ht and waist circumference (WC) were measured. Routine serum biochemical indicat-ors were tested.3ml peripheral venous blood was saved at the condition of-80℃for the detection of ox-LDL, another3ml stored at-80℃for extraction of genomic DNA. The statistical processing of data we used SPSS17.0.Results:1、The comparison of baseline date in two groupsCompared with the control group C allele frequency, the proportion of CC+GC genotypes, male proportion, CHD family history, smoking history, diabetes, metabolic syndrome, and levels TG, LDL-C, WBC, SCr, UA, FBG, ox-LDL are significantly higher in disease group (p<0.05); but HDL-C, the age is lower (P<0.05).2、At the gene level of rs281432Between the two groups, ICAM-1gene rs281432polymorphism analysis show-ed:the genotype and allele frequency distribution are significantly different, the disease group have higher (CC+GC)/GG genotype ratio than control group, the differe nttce has statistical significance (X2=8.385,P=0.004); Disease group CC/GG genotype ratio is significantly higher than control group (P<0.05). Disease group C allele frequency is significantly higher than control group (X2-8.996, P=0.003)3、The comparison of baseline date in different genotypes of rs281432.GC+GG genotypes in disease group with significantly higher serum ox-LDL, TC, LDL, FBG levels than the GG genotype patients (P<0.05)4、Gene rs281437polymorphism analysisThere is no significant difference at the proportions of the genotypes, allele frequency and distribution between the two groups, but the level of TG and PLa in CC genotype patients is significantly lower than CT genotype patients (P<0.05)5、Logistic regression analysisThe results showed that:male, history of diabetes, elevation of LDL-C and ox-LDL levels and rs281432gene variants are risk factors for coronary heart disease. People with CC or CG genotype suffering from the1.666times the risk of CHD than those with GG genotype.Conclusion:Male, history of diabetes, elevation of LDL-C and ox-LDL and ICAM-1gene rs281432mutation are risk factors for coronary heart disease (CHD). ICMA-1gene rs281432polymorphism is associated with serum levels of FBG, ox-LDL, LDL, TC levels. ICAM-1gene rs281432C allele is still one of the independent risk factors for CHD. |
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