CETP TaqIB And1405V Gene Polymorphisms And Atorvastatin Treatment Efficacy With Coronary Heart Disease

Objectives:Cholesteryl ester transfer protein and its main function are to promote the exchange and balance of cholesterol esters and triglycerides in plasma lipoproteins. Related to HDL and LDL particle size and lipid composition plays a key role in reverse cholesterol transport. Recently, The research results are still controversial whether Plasma CETP levels and HDL-C and increase HDL-C levels inhibit CETP activity can reduce CHD prevalence rate.This study investigated the polymorphism of the China Tianjin crowd CETP gene TaqIB and1405V sites, CETP and lipid metabolism and the risk for CHD research; Analysis of CETP gene TaqIB and the I405V the chain relationship.CETP TaqIB and the I405V different genotypes observed statins in patients with coronary heart disease lipid role.Methods:1. Select748patients who were admitted to coronary angiography in Tianjin Chest Hospital from Sep2010to Oct2011.According to the results of coronary angiography classified into CHD group, n=472,normal control group,n=276.Application of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to the total research object TaqlB and I405V gene loci polymorphisms were analyzed,at the same time by using enzyme-linked immunosorbent (ELISA) for determining the content of the serum CETP,Analysis of the CETP and lipid metabolism and the correlation of CHD risk.SHEsis software was applied to the CETP gene I405V and TaqIB chain relation is analyzed.2. CHD patients admitted to hospital for atorvastatin calcium20mg, every night. After treatment for3months, observation of statins to carry the CETP TaqIB and I405V different genotypes of coronary heart disease in the blood lipid regulation.Results:Tianjin area the Han population CETP gene TaqIB sites B2allele and I405V sites V allele frequency are0.409and0.402, basically similar to the B2allele frequencies at home and abroad(B20.34～0.46)(V0.32-0.486).CHD group B1B1, B1B2, B2B2genotype compared serum HDL-C levels upward trend, the difference was statistically significant (P=0.043). CHD group in II, IV, VV genotype comparison, serum TC, HDL-C, LDL-C level upward trend, no statistically significant difference (P>0.05). CHD group and the control group CETP TaqIB genotypes and allele distribution differences were not statistically significant (P>0.05). CHD group and control group1405V genotype distribution difference was statistically significant (P<0.01), allele was no significant difference (P>0.05) Two loci in linkage disequilibrium (r2=0.202), and two loci may have occurred restructuring or mutation (D’=0.459). Two loci haplotypes were not statistically significant(P>0.05). Diabetes, smoking history, serum LP (a), ApoB risk factors for CHD, Serum HDL-C protection factors for CHD.B1B1, B1B2, the B2B2genotype LDL-C, Lp (a) level was B1B1drops are the most obvious,the rate of decline are22.76%and26.31%,but the difference did not reach statistical significance(P>0.05). Genotype Ⅱ and Ⅳ+ⅤⅤ genotype compared with serum LDL-C, TC level Ⅱ genotype was up to32.74%and20.63%(P<0.01).Serum HDL-C, ApoAl level Ⅳ+ⅤⅤ genotype respectively increased up to14.12%(P<0.05) and15.72%(P0.01).Conclusions:Tianjin Han population CETP TaqIB and I405V two points genotype and allele frequency distribution of the basic similarity with the rest of the country and abroad crowdTianjin Han population. CETP TaqIB and I405V mutation and the incidence of CHD risk.CETP TaqIB and I405V gene locus linkage disequilibrium analysis, the results show that the two loci linkage disequilibriums. CETP TaqIB and I405V gene polymorphisms and atorvastatin calcium CHD patients with lipid regulating role, TaqIB B1allele and the I405V I allele better lower TC, LDL-C level of treatment effect,I405V gene V allele have a better increase in HDL-C, ApoAl level of treatment effect.