Correlation Of The Genotype And Mutation Of HBV As Well As C-myc Protein In HCC

Objective: To comprehend the correlation of HBV genotype,1762/1764and1896mutation as well as c-myc protein in HCC for further investigation about the pathogenesis and theoretical basis about prevention, cure and early diagnosis of HCC.Methods: HBV genotype and mutation of80samples including normal, chronic HBV carrier, chronic Hepatitis B, hepatitis B Induced cirrhosis and HBV-associated HCC were tested by NAAT and sequencing method, sequenced by the auto genotype analyzer and tested by the quantitative method and chromas program. The quantization of HBV-DNA was detected by FQ-PCR. Expression of c-myc protein in above-mentioned5groups liver tissues was detected by immuno-histochemistry staining method and results were judged by Semi-quantitative integration. Used ELISA tested the HDVM of the HBV serums antigen antibody reaction and analyzed the result by statistical methods. All the testing data were processed statistically by SPSS17.0statistical software package.Results:1、Among the78chronic HBV infectors, there were31cases genotype B with the proportions39.7%(31/78), while47cases C with60.3%(47/78). There were no other genotypes found in this research. Genotype C is the main HBV genotype in Yunnan province. The proportion of Genotype C in group ASC, CHB, LC and HCC was42.9%(6/14),50.0%(15/30),64.3%(9/14) and85.0%(17/20), and the discrepancy had statistical significance(χ2=8.29, p=0.04). While the proportion of genotype C was overwhelming in group LC and HCC, but the discrepancy of distribution in the two groups had no statistical significance(p=0.161). Combining of the two groups(26/34) was compared with group CHB and ASC(21/44), and the discrepancy had statistical significance(χ2=6.62, p=0.01). The proportion of genotype C in group LC and HCC was higher than in group CHB and ASC.2、There were51cases with BCP mutation and44PC mutation in78chronic HBV infectors. The proportion of BCP mutation with genotype C in HBV infectors was50.0%(3/6),86.7%(13/15),88.9%(8/9) and94.1%(16/17), while PC mutation was33.3%(2/6),80.0%(12/15),88.9%(8/9) and88.2%(15/17), both of the two mutation were more than HBV infectors with genotype B, and the discrepancy had statistical significance (p<0.05) except group ASC.3、Expression of c-myc protein in group HCC was strongest with positive rate75.0%, next came group LC(64.3%), CHB(53.3%) and ASC(42.9%), and the discrepancy among groups had statistical significance(p<0.05). Combining of group LC and HCC(70.6%) was compared with group CHB and ASC(50.0%), and the discrepancy of c-myc protein expression in the two had statistical significance (p<0.05). Strong positive cases of c-myc protein expression in group LC and HCC were more than group CHB and ASC.4、Positive rate of c-myc protein expression in chronic HBV infectors with genotype C in group ASC, CHB,LC and HCC was83.3%(5/6),80.0%(12/15),88.9%(8/9) and88.2%(15/17), which was higher than in genotype B, that was12.5%(1/8),26.7%(4/15),20.0%(1/5)and0%(0/3). The discrepancy had statistical significance(P<0.05). The proportion of HBV infectors with positive BCP mutation and positive c-myc protein expression in the four groups was66.7%,75.0%,77.8%and86.7%, were higher than HBV infectors with negative BCP mutation, while proportion of positive PC mutation was50.0%,68.8%,77.8%, and66.6%, were higher than HBV infectors with negative PC mutation except group ASC, But the discrepancy had no statistical significance (p>0.05).Conclusion:1、There are genotype B and C, mainly genotype C existing in chronic HBV infesters (including chronic HBV carriers, chronic Hepatitis B, Hepatitis B Induced Cirrhosis and HBV-associated HCC) in Yunnan Province, and there were no other genotypes found. There is a connection between the genotype of chronic HBV infectors and the clinical spectrum of disease, genotype C has connection with the relatively serious liver disease(Hepatitis B Induced Cirrhosis and HBV associated with HCC).2、There were more BCP and PC mutation with genotype C than B in every group.3、Expression of c-myc protein in group HCC was strongest, next came group LC, CHB and ASC. Strong positive cases of c-myc protein expression in group HCC and LC were more than group CHB and ASC.4、Positive rate of c-myc protein expression in chronic HBV infectors with genotype C was higher than genotype B. There was obvious BCP and PC mutation in chronic HBV infectors with positive c-myc protein expression. Correlation exists between c-myc protein expression and genotype C, whether c-myc protein expression is related with BCP and PC mutation still need further research by extending the numbers of cases.5、HBV genotype C, BCP and PC mutation as well as c-myc protein are related with the occurrence and development of HCC. But whether there are cooperation among them, whether they are related with disease development, and the definite mechanisms that they advance the occurrence of HCC are still need further research by extending the numbers of cases and expanding research area.