The Study On Correlation Between RhoC、Bmi-1、uPA、PAI-1Invasiveness、Migration And Prognosis Of Esophageal Squamous Cell Carcinoma

Objective: To investigate the expression of RhoC (ras homologue)、 Bmi-1（B-cell-specific Moloney murine leukemia virus integration site1protein）、 uPA(urokinase-type plasminogen activator) and PAI-（1plasminogen activator inhibitor type-1）in esophageal squamous cell carcinoma (ESCC) and its clinical pathological significance.Methods: The expression of RhoC、Bmi-1、uPA and PAI-1Protein in specimens ofesophageal squamous cancer was detected by immunohistochemistry. The relationship ofRhoC、Bmi-1、uPA and PAI-1expression was evaluated using χ~2testing and COXregression model and Kaplan-Meier survival curve analysis. Results: The positive rates ofRhoC、Bmi-1、uPA and PAI-1Protein expression in esophageal squamous carcinoma washigher than those in normal esophageal tissues (72.5％vs30％,both P<0.05;78.8％vs5％,both P<0.05;75％vs45％;75％vs10％, both P<0.05). The expression of RhoC wasrelated to invasion depth、lymph node metastasis and differentiation degree (P<0.05). Onthe other hand, not related to patient’s age、 tumor size、nationality、pathological typesand gender(P>0.05). The expression of Bmi-1and uPA and PAI-1was related to invasiondepth, lymph node metastasis (P<0.05). however, not related to patient’s age、 tumorsize、nationality、differentiation degree、pathological types and gender(P>0.05). Theexpression of RhoC was positively related to that of Bmi-1(r＝0.338, P<0.05). Theexpression of uPA was positively related to that of PAI-1(r＝7.092, P<0.05). Thefive-year overall survival rate for all the patients was20％(1680). Univariate survivalanalysis suggested that the five-year survival rate for patients with positive expression ofRhoC was higher than for patients with negative expression of RhoC though the differencewas not significant(P>0.05); the five-year survival rate for patients with positiveexpression of Bmi-1was higher than for patients with negative expression of Bmi-1 though the difference was not significant(P>0.05); the five-year survival rate for patientswith positive expression of uPA was higher than for patients with negative expression ofuPA though the difference was not significant(P>0.05); the five-year survival rate forpatients with positive expression of PAI-1was higher than for patients with negativeexpression of PAI-1though the difference was not significant(P>0.05). Cox multivariateanalysis illustrated that invasion and metastasis were independent prognostic markersabout survival of ESCC patients (both P<0.05). Conclusion: The overexpression ofRhoC、Bmi-1、uPA and PAI-1in ESCC enhances the invasion and metastasis. They mayserve as a prognostic factor to show biologic behavior of esophageal squamous cellcarcinoma.