| EMT（epithelial-mesenchymal transition, EMT） is a biologicalprocess characterized with the loss of the epithelial cell polarity and phenotypeand gain of the mesenchymal phenotype. Epithelial cells can decrease their abilityof adhesion and increase their ability of invasion and migration. EMT plays a keyrole in the development of tumor. The lower expression of E-cadherin is amolecular indicators.The carboxyl-terminal binding protein1(CtBP1) is a newlydiscovered cell factor and an evolutionarily conserved transcriptional co-inhibitor.CtBP1combined with inhibition of transcription factors Zeb1, Snail inhibits theexpression of E-cadherin,promotes the epithelial mesenchymal transition andstimulates the formation and development of tumours. At the same time, renal cellcarcinomas are closely related to genetic factors.The study regards Renal CellCarcinoma as an object, detects CtBP1and E-cadherin protein expression indifferent histological types of renal cancer and normal tissues, explores theoccurrence, development, invasion, and metastasis of renal cell carcinoma and the relationship, seeks potential clinical significance and prognostic value ofmolecular indicators for the clinical diagnosis and treatment, provides a scientificbasis and thinking way for diagnosis, treatment and prognosis of renal cellcarcinomas. Methods：The expression of CtBP1and E-cadherin in62cases ofRenal Clear Cell Carcinoma,18cases of Papillary Renal Cell Carcinoma and15cases of Chromophobe Renal Cell Carcinoma and95cases of adjacent noncancerous normal tissue were detected by immunohistochemistrystreptavidin-perosidase (S.P). Evaluate the expression difference in differenthistological types of renal carcinoma and the correlation of CtBP1,E-cadherinexpression with clinic pathological characters including age, sex,tumor grade,stage and lymphatic metastasis. SPSS17.0was applied to analyze the results ofexperiment and p<0.05was considered as statistical significiance. Results：BothCtBP1and E-cadherin express in epithelial cells of kidney tubular (derived cellsof renal cell carcinoma) of paracancerous tissues of different histological types ofrenal cell carcinoma.The expression of CtBP1is partly missing in cancertissues.Great difference of CtBP1shows between renal cell carcinomas andparacancerous tissues(p＜0.001). The positive rate in Chromophobe Renal CellCarcinoma(CRCC), Renal Clear Cell Carcinoma(RCCC) and Papillary Renal CellCarcinoma (PRCC)are6.67%（1/15），33.87%（21/62）, and44.44%（8/18）respectively. Difference expression of CtBP1exists between RCCC and CRCC(p=0.036), PRCC and CRCC(p=0.015).The expression of E-cadherin is also partlymissing. Great difference of E-cadherin also shows between renal cell carcinomas and paracancerous tissues(p＜0.001). The positive rate in Chromophobe RenalCell carcinoma, Papillary Renal Cell Carcinoma, Renal Clear Cell Carcinoma are93.33%（14/15）,16.67%（3/18）,20.97%（13/62）respectively. Great differenceof E-cadherin exists between RCCC and CRCC(p＜0.001),PRCC and CRCC(p＜0.001). The expression of CtBP1and E-cadherin have relation with grade,tumorstage and lymphatic metastasis, but no relation with age and sex in Renal ClearCell Carcinoma. In Renal Clear Cell Carcinoma and Papillary Renal CellCarcinoma,the expression of CtBP1has no correlation with the expression ofE-cadherin. In Chromophobe Renal Cell Carcinoma, the expression of CtBP1hascorrelation with the expression of E-cadherin.(r=-1.000，p＜0.001).Conclusions:CtBP1and E-cadherin are differentially expressed in different histological type ofrenal carcinomas. It’s a potential marker in clinic pathological diagnose andprognosis of Renal Cell Carinoma. CtBP1may be one of major regulator factorswith E-cadherin in Chromophobe Renal Cell Carcinoma. |
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