| Background Tumor resolve and ease are regarded as the standard oftumor’s therapeutic effect. At present, although it is satified inshort-term effects of tumor, the most important factor to influence theprognosis of the tumor is its recurrence, which is the major cause of lowfive year survival rate. However, the theory of cancer stem cell has aprofound significance in tumor’s diagnosis and treatment. The theory ofCancer Stem Cells deems that tumor cells originate from the stem cells in the tissue,which the most important content is that cancer consists of a group of cells withheterogeneity, however just a minority of it having the ability ofself-renewal, immortalization and mutiple differentiation potential arestarter cells and maintain the continuous growth of tumor, and the othercells only have the relative ability of proliferation and differentiation.During the stages of the tumor’s generation, development and metastasis,cancer stem cells paly an essential role. It becomes the first importantwork to find out the accute and reliable molecular marks in the field oftumor stdy. With the further reseach of cancer stem cells, more and morecancer stem cells have been isolated and identitied. At present, more andmore researches have confirmed liver cancer stem cells exist in theHepatocellular Carcinoma, however specialists have not reached an agreementon which is the CSC of the Hepatocellular Carcinoma. Therefore, there is aprofound signifiacance to find out the real LCSC. Now, the reseach of LCSC have been in exploratory stage, and most ofit has focused on the CD133, CD44, CD90and side population cells. CD133had widely been studied as the molecular mark of rectal carcinoma. Laterreseach had found that the CD133+cells in Hepatocellular Carcinoma showedthe features of stem cell. Another transmembrane protein of liver cancercells, CD105, involves in the production process of neovascularization,which is closely related with metastasis of Hepatocellular Carcinoma.Objective The objective of this study is to compare the expression of CD105andCD133in cell membrane of Human Hepatocellular Carcinoma cell line and thediffrence of biological characters among the subpopulations in vitro, andto explore preliminarily the the characterristics of stem cells ofCD105+CD133+subpopulation.Methods HepG-2cells were cultured in DMEM. Flow cytometry（FCM）was used todetect the expression of CD105and CD133in HepG-2cells, and then sortedrespectively out the CD105+CD133+, CD105-CD133+, CD105+CD133-andCD105-CD133-cells. The ability of proliferation and invasion of cells obtained fromFACS were determined by the Cell Counting Kit-8(CCK-8) and Transwell assay. Andthe self-renewal capacity of the cells were observed by colony forming assay in vitro.Results The rates of the four kinds of cells were respectively99.57%,0.2%,0.2%and0.03%. The study of proliferative activities showed that the CD133+cells growmore quickly than that of CD105+and no-sorted cells. The number of colonies formedby CD133+cells were more than CD105+cells and no-sorted cells. Whereas, themetastatic capacity of CD105+cells observed by Transwell assay was higher than thatof the CD133+cells and no-sorted cells. Conclusions CD105+cells in human hepatocellular carcinoma cell line Hepg-2related with its ability of invasion and that of CD133+cells correlated with its ability ofproliferative. CD105+CD133+cells are a subpopulation of HepG-2cells with stemproperties and uniting CD105with CD133may be the candidate surface marker forliver cancer stem cells. |
PDF Full Text Request