Synthesis And Characterization Of Chitin And Its Derivatives Hybrid Membrane And The Study Of Its Woundhealing Functions

Objective: Trauma treatment has become one of the most rapid subjects asmodern medicine development, the research of trauma treatment materials is animportant part about this subject. The ideal guaiac materials have goodbiocompatibility, biodegradability, preventing infection and antibacterial and healingeffects, but currentlly the clinical materials often exist different defects, and they aredifficult to meet the requirements. The research shows that chitin or chitosanderivatives have much research value, the performances often show their good resultsand have the potential to become the model lignum biomaterials. But single chitin donot have all the excellent properties, some materials of water-soluble characteristicsoften limit its application. Therefore, this experiment fixes on the synthesis guaiaceffects of chitin and its derivatives by the preliminary study on preparation,physicochemical properties and biological functions of the hybrid membranes ofchitin and its derivatives, expected to provide a new kind of material and a nice wayof burn treatment and also provide some theoretical basis for the further use andresearch of chitin and its derivatives.Methods:1. Preparations of five kinds of chitin hybrid membranes by differentways. Examine the physicochemical properties of the membranes including themechanical strength, water absorption and determination, then screening thediaphragms which meet the subsequent experimental conditions, CT-HCTS andCT-CMCTS.2. Examine the properties of CT-HCTS and CT-CMCTS, includinginfrared spectroscopy, ash, surface evaporation rate, thickness.3. Evaluated CT-HCTSand CT-CMCTS by hemolysis experiment, prickles test, acute systemic toxicity test,cytotoxicity and biocompatibility of biological safety experiments.4. Prepare the deeppartial thickness scald in New Zealand rabbits and setting negative control group, experimental group1(CT-HCTS), experimental group2(CT-CMCTS) and positivecontrol group (hemostatic wound-healing yarn). Observe and record the decrustationtime and healing rate, tissues were harvested respectively in7d,14d,21d,28d andthen examined pathologically. Determination of hydroxyproline and the expression ofTGF-β1of by ELISA.Results:1. CT-HCTS and CT-CMCTS were prepared successfully, and meet thestandard requirements of hemolysis, systemic acute toxicity experiments and cellstoxicity and skin irritation have no adverse reaction, the indicators are in line with thesafety standards of biomedical materials.2. Results in the treatment of deep partialthickness scald in New Zealand rabbits showed, compared with the negative controlgroup, the experimental group can promote wound decrustation and healingsignificantly (P <0.05), and increased the expression of hydroxyproline and TGF-β1(P <0.05), and the peak in advance. The difference between the experimentalgroups did not significantly (P>0.05); the healing effect of positive control group ishighly significant (P <0.01); the healing effect of experimental groups is weak andhave significant differences compared with the positive control group(P <0.05).Conclusion: The two kinds of hybrid membranes of CT-HCTS and CT-CMCTSwhich have potential of becoming new guaiac material have good biological safetyand the healing of wound can been effectively promoted, the expression of TGF-β1,hydroxyproline are increased and the amount of peak is ahead of time according to thecontrol group.