The Study For Immunological Mechanisms Of Human Anaplastic Large Cell Lymphoma BALB/C Mouse Model

Objective：To establish the BALB/c mouse model with human anaplastic large celllymphoma (ALCL) cell line Karpas-299, and to explore the immunological mechanisms offormation of tumor.Methods: With the Cytoxan(CTX) pretreatment, Karpas-299cells were injected to armpitswith0.5m（l1×107/ml）, every three days strengthen, once or twice in all. The CTX controlgroup were set up. After the tumor formation, peripheral blood was collected to detect Tand B lymphocyte subgroup proportion by flow cytometry (FCM).Results:1. Tumor tissues for HE staining showed nodular pattern of tumor cells with largeor medium cell size, abundant cytoplasm, light staining nucleolus, and mitotic figures. Thetumor cells usually express ALK, EMA and CD30, and without expressing of CD20andCD45RO, Ki-67>80%2. In tumor group, the proportion of CD3, CD8, CD19positive cellswere significantly lower than that of the control group (P<0.05). In group without tumor，the proportion of CD8, CD19positive cells were significantly lower than that of thecontrol group, the proportion of CD20positive cells were significantly higher than that ofthe control group(P<0.05); In group without tumor, compared with the tumor group, theproportion of CD3, CD4, CD8, CD20positive cells were significantly higher than that ofthe control group (P<0.05).3. In control group, on third day, compared with before theinjection of CTX, the proportion of CD3positive cells was raised, the proportion of CD19positive cells was descended; on the tenth day, All of the detected immune indexes arelower than before the injection of CTX，a decline in percentage of CD8, CD19, CD20positive cells have significant (P <0.05); on the24th day, with the exception of CD8,CD19, CD20positive cell ratio is slightly lower than before the injection of CTX (P <0.05), the proportion of CD3, CD4positive cells have no significant difference comparedwith before the injection of CTX (P>0.05). Conclusions：The animal models were successfully established. Immune suppressioncaused by Cytoxan plays a determining role in the beginning of tumor formation, the rapidgrowth of the tumor may be associated with the decreased CD3+and CD8+cells.