Effects Of HPS On Lipid Metabolism In The NAFLD SD Rats Induced By High-fat Diet

Objective：The model of non-alcoholic fatty liver disease (NAFLD) was establishedby high-fat diet, and intervened with Hedysarum polybotys saccharide (HPS) or positivedrug. The influence of liver enzyme and Lipid Metabolism，as well as the expressions of FAS,SCD-1, CPT-1gene of rats in each group were detected and analyzed. The result of this studywould provide us new understandings and knowledges about the mechanisms and therapeuticefficacies of HPS on NAFLD.Methods：36SD rats were selected and divided randomly into the control group (10rats) and high fat diet group (26rats) after a week of adjustable feeding. The rats in thecontrol group were given fundamental feed while the rats in high fat diet group were fed withhigh fat diet. Rats took food and water freely during experimental period. Two rats in eachgroup were selected randomly and killed for pathological section to make sure that modelswere made successfully after8weeks. The rats in high fat diet group were divided into modelgroup (8rats), positive drug group (8rats) and HPS group (8rats). Rats in HPS groupwere fed with HPS (150mg per kg every day) by gavage and in the positive drug group werefed with vitamin E (800mg per kg every day) while rats in model group and blank controlgroup were given distilled water for8weeks. Then the general condition of each group andpathologic changes of liver were observed and the level of serum ALT, AST, TG, TC, HDLand LDL were detected. The level of FAS, SCD-1and CPT-1gene expression wasquantitatively tested with real-time PCR.Results：Compared with that of blank control group, the level of serum ALT, AST, TC,TG, LDL-c of model group rats were significantly higher, the level of HDL-c of model grouprats was significantly lower, statistically significant difference (p<0.01). The expression ofFAS and SCD-1gene were obviously higher while the expression of CPT-1gene wasobviously lower, statistically significant difference (p<0.01). With drug intervention,compared with model group，HPS and vitamin E were useful to decline the level of serumALT, AST, LDL-c, TC and TG obviously, increase the level of HDL-c, drow-regulated theexpression of FAS and SCD-1gene and up-regulated the expression of CPT-1gene obviously,statistically significant difference (p<0.01). Conclusion：We could reach the following conclusions by analyzing the mechanismsand therapeutic efficacies of HPS on NAFLD:1. HPS has a positive effect on improving hepatocyte injure caused by lipidaccumulation during the simple fatty degeneration of NAFLD by declining index of serumenzymes.2. HPS has a positive effect on treating lipid accumulation by declining the level of TG,TC and LDL and increasing HDL level.3. HPS has a positive effect on depressing lipid synthesis and improving β-oxidation offatty acid by drow-regulating the expression of FAS and SCD-1gene and up-regulating theexpression of CPT-1gene.