Effect Of Autocrine Motility Factor On Biological Property In Human Dermal And Keloid Fibroblasts

Objective: To observe the effect of autocrine motility factor(AMF) onthe biological property，proliferation, anti-apoptosis and migration of thecultured human dermal fibroblasts and human keloid fibroblasts.Methods: The patients which underwent circumcision（n=10） andkeloid excision（n=9） between2011November and2012June wererespectively nipped specimens of the dermis and keloid organizations usedfor primary culture of the fibroblasts. These were divided into experimentalgroup(AMF group) and control group. Cell proliferation, cellularanti-apoptosis and cell cycle, and migration were respectively determined byMTT assay, flow cytometry and Transwell Chambers to explore thebiological activities of AMF and fibroblasts between the two groups.Results: The available human dermal fibroblasts（n=8） and humankeloid fibroblasts（n=4）could be achieved by primary culture after4to5weeks. the abilities of proliferation of the experimental group (0.709±0.028；0.800±0.018) is higher than that of the control group(0.610±0.024；0.705±0.018)(t=9.13, p＜0.001; t=16.086, p＜0.001) the apoptosis rate of the experimental group(5.11±0.30;4.93±0.19)%is lower than that of thecontrol group(13.40±0.64;9.63±0.37)%(t=30.94, p＜0.001; t=26.221, p＜0.001), and migration of the experimental group(71.9±4.5;161.2±5.3) ishigher than that of the control group(50.2±5.9；122.7±4.6)(t=5.35, p <0.001; t=0.001, p <0.001). In mock group, the anti-apoptosis and movementability of human keloid fibroblasts are higher than that of the dermalfibroblasts (p <0.05).Conclusion: AMF could induce cell proliferation, anti-apoptosis andmigration of human dermal fibroblasts and human keloid fibroblasts, whichwould contribute to the development of keloid. That suggested AMF wasassociated with keloid in characteristics of oncology.