The Correlation Of Apoe Gene Polymorphism And The Locality And Size Of Cerebral Infarction

objective:Though CT/MRI scan and calculating thelocality and infarction volume of each patient with cerebral infarction(CI), and test apolipoprotein E (ApoE) genotype of the patients and thecontrol group.Discuss the correlation of ApoE gene polymorphism andthe locality and size of cerebral infarction, to provide a new basis for theCI genetic research and clinical diagnosis and treatment.Methods:In all,162patients with CI and the control groupcomprised120healthy human beings included.Taking2ml peripheralvenous blood to extract DNA, and used for the preparation of genomicDNA template, detection ApoE genotype by the multiple polymerasechain-ligase detection reaction (PCR-LDR) technology. CT/MRI Headscan calculating each patient infarction location and the size of theinfarcts,Analysis the different parts of the ApoE genotypes and thedifferences of alleles distribution with the control group,and relationshipwith the size of the infarct volume.SPSS19.0software was used toanalyze these date.Result:①there was no significant difference between CI group andthe control group about individual gender composition, age, and BMI. In CI group and the control group,all have were detected withE3/3, E3/4, E3/2, E2/4, E4/4and E2/2,6kinds of phenotype. And E3/3isthe highest proportion of homozygous, the frequency is70.4%and73.3%respectively.Statistics found that there is no significant differencebetween the two groups of6kinds of genotype distribution in individuals.(χ~2＝4.476，P=0.483).In CI group, the frequency of allele ε3、ε4、ε2was83.0%,9.6%and7.4%respectively.While In the control group, thefrequency was83.3%、11.3%、5.4%.There was no statistical differencebetween two groups in (χ~2＝1.214， P=0.545). There was nostatistical difference in the frequency of three allele distribution inhemispheric infarction,basal ganglia and thalamus infarction,cerebellumand brainstem infarction, multiple infarction(χ~2=10.211， P=0.806).Further compared the genotype with control group with differentparts, there were no statistical differences (P>0.05).What’smore,compared the frequency of the three allele in each part, thedifference is meaningless（χ~2=3.784，P=0.706）,compared with thecontrol group, the allele frequency of each part had no statisticaldifference (P>0.05).④The average infarction volume of CI patients withE4/X is15.7士2.3（cm3）,while the average infarction volume with E3/3is10.2士2.1（cm3）（q=8.09，P<0.01）,and with E2/X is9.5士1.8（cm3）（q=5.18，P<0.01）,there was a significant difference. While there hadno significant difference in average volume of infarction with E3/3和 E2/X (P>0.05).In these patient with E4/X of Massive CerebralInfarction was accounted for56%, more than E3/3of28.1%（χ~2=7.224，P=0.007）, E2/X of26.3%（χ~2=3.877，P=0.048）, the difference isstatistically significant.Conclusions:①ApoE genotype polymorphism has no correlationwith the incidence of CI, and the ApoE gene may not be a risk factor forcerebral infarction.②There is no correlation of ApoE genotypepolymorphism and CI location.③Maybe ε4associated with infarctionvolume, with ε4are more prone to massive cerebral infarction, whichbigger than withε2、ε3.